高遷移率族蛋白1(HMGB1)單克隆抗體
Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)
HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein
- 編號UMAA399Ra21
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)-
- Ig亞型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 -
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 326 ¥ 760 ¥ 1086 ¥ 2715 ¥ 10860
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對HMGB1的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別HMGB1。
用法
免疫印跡：0.2-2μg/mL;1:500-5000
免疫組織化學：5-20μg/mL;1:50-200
免疫細胞化學：5-20μg/mL;1:50-200
最佳稀釋倍數(shù)最終由用戶決定。
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPA399Ra01 | 高遷移率族蛋白1(HMGB1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA399Ra01 | 高遷移率族蛋白1(HMGB1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA399Ra01 | 高遷移率族蛋白1(HMGB1)多克隆抗體 | WB; IHC |
| ULAA399Ra81 | 高遷移率族蛋白1(HMGB1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA399Ra71 | 高遷移率族蛋白1(HMGB1)多克隆抗體(生物素標記) | WB |
| UMAA399Ra21 | 高遷移率族蛋白1(HMGB1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA399Ra | 高遷移率族蛋白1(HMGB1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA399Ra | 高遷移率族蛋白1(HMGB1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA399Ra01 | 高遷移率族蛋白1(HMGB1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
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| Oxidative Medicine and Cellular Longevity | Diabetes-Induced Oxidative Stress in Endothelial Progenitor Cells May Be Sustained by a Positive Feedback Loop Involving High Mobility Group Box-1 [Journals: Omcl] |
| Int Immunopharmacol | Hesperidin ameliorates lipopolysaccharide-induced acute lung injury in mice by inhibiting HMGB1 release [PubMed: 25724384] |
| Journal of Immunotherapy | Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity [PubMed: 25962106] |
| Atherosclerosis | Retinoic acid inhibits tissue factor and HMGB1 via modulation of AMPK activity in TNF-α activated endothelial cells and LPS-injected mice [PubMed: 26116962] |
| PLOS ONE | Plasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in China [PubMed: 26317615] |
| Int Immunopharmacol | Betulin attenuates lung and liver injuries in sepsis [PubMed: 26644168] |
| journal of cardiochoracic surgery | High mobility group box 1 gene polymorphism is associated with the risk of postoperative atrial fibrillation after coronary artery bypass surgery [PubMed: 26109393] |
| Int Immunopharmacol | Glycyrrhizin reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and endotoxemic mice by p38/Nrf2-dependent induction of HO-1 [PubMed: 25812767] |
| World J Gastroenterol | Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitro [PubMed: 26167076] |
| Neurotoxicology | Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats [PubMed: 25845300] |
| Journal of Neuroimmunology | HMGB1 expression patterns during the progression of experimental autoimmune encephalomyelitis [PubMed: 25773152] |
| J Neuroinflammation | HMGB1 release triggered by the interaction of live retinal cells and uveitogenic T cells is Fas/FasL activation-dependent [PubMed: 26394985] |
| PLoS One | Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-1 Pathway to Induce Inflammation Response [PubMed: 25793984] |
| Neuroscience | Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice [PubMed: 26363151] |
| Cancer Biomarkers | Serum levels of HMGB1 have a diagnostic role in metastatic renal cell cancer [Pubmed:27062570] |
| International Immunopharmacology | Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways [Pubmed:27179304] |
| SpringerPlus | Ethyl pyruvate?attenuated?coxsackievirus?B3-induced?acute?viral?myocarditis?by?suppression?ofHMGB1/RAGE/NF-ΚB?pathway. [Pubmed:27026909] |
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| Molecular?Immunology | HMGB1 blockade differentially impacts pulmonary inflammation and defense responses in poly (I: C)/LPS-exposed heart transplant mice [pubmed:27387278] |
| Oxidative Medicine and Cellular Longevity | Reduced HMGB 1-Mediated Pathway and Oxidative Stress in Resveratrol-Treated Diabetic Mice: A Possible Mechanism of Cardioprotection of Resveratrol in … [pubmed:27833703] |
| BioMed?Research?International? | High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A [pubmed:27579314] |
| Cardiology | The role of high mobility group box 1 protein in interleukin-18-induced myofibroblastic transition of valvular interstitial cells [pubmed:27395056] |
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| Immunol Res. | Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells. [pubmed:28108937] |
| Biomed Pharmacother. | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. [pubmed:28122311] |
| Sci Rep.? | Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats. [pubmed:28373667] |
| Integrative Cancer Science and Therapeutics | The expression of HMGB1 and the receptor for advanced glycation end products (RAGE) in epithelial ovarian cancer [pdf:ICST-4-232.pdf] |
| Journal of Food and Drug Analysis | Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of?Cinnamomum osmophloeum?Kanehira in endotoxin-induced … [S1021-9498(17)30081-9] |
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| Scientific Reports COMMUNICATIONS | Mineral particles stimulate innate immunity through neutrophil extracellular traps containing HMGB1 [pubmed:29192209] |
| Biochemical and Biophysical Research Communications | Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release [pubmed:29146189] |
| Pulmonary Pharmacology & Therapeutics | HMGB1 promotes HLF-1 proliferation and ECM production through activating HIF1-a-regulated aerobic glycolysis [pubmed:28571757] |
| Biomedical Research | Correlation between high mobility group box 1 protein, tumor necrosis factor-α, and acute coronary syndrome [ISSN 0970-938X] |
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| Cellular physiology and biochemistry | Macrophage Inflammatory Protein-2 in High Mobility Group Box 1 Secretion of Macrophage Cells Exposed to Lipopolysaccharide. [pubmed:28662496] |
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| Journal of Molecular?Cell?Biology | S1 PR 3 is essential for phosphorylated fingolimod to protect astrocytes against oxygen‐glucose deprivation‐induced neuroinflammation via inhibiting TLR 2/4‐NF κB?… [Pubmed:29536648] |
| Frontiers in Immunology | Glycyrrhizin Protects Mice against Experimental Autoimmune Encephalomyelitis by Inhibiting HMGB1 Expression and Neuronal HMGB1 Release [Pubmed:30013568] |
| Oncoimmunology | Bromodomain inhibition exerts its therapeutic potential in malignant pleural mesothelioma by promoting immunogenic cell death and changing the tumor immune?… [Pubmed:29399399] |
| Cancer Immunology, Immunotherapy | Tumor lysate-based vaccines: on the road to immunotherapy for gallbladder cancer [Pubmed:29600445] |
| Cytokine | Porcine reproductive and respiratory syndrome virus induces concurrent elevation of High Mobility Group Box-1 protein and pro-inflammatory cytokines in?… [Pubmed:29895396] |
| Frontiers in Neurology | Ursolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1/Toll-Like Receptor 4/NFκB?… [Pubmed:29867706] |
| Biomedicine & Pharmacotherapy | Oridonin enhances the cytotoxicity of 5-FU in renal carcinoma cells by inducting necroptotic death [Pubmed:29958141] |
| Inflammation?Research | Luteolin activates ERK1/2- and Ca-dependent HO-1 induction that reduces LPS-induced HMGB1, iNOS/NO, and COX-2 expression in RAW264.7 cells and?… [Pubmed:29497773] |
| Journal of?Food?and?Drug?Analysis | Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice [Pubmed:29389558] |
| Biochemical and Biophysical?Research?Communications | Sirt1 S-nitrosylation induces acetylation of HMGB1 in LPS-activated RAW264. 7 cells and endotoxemic mice [Pubmed:29680657] |
| Neurological Sciences | Thrombomodulin, alarmin signaling, and copeptin: cross-talk between obesity and acute ischemic stroke initiation and severity in Egyptians [Pubmed:29637447] |
| International Journal of Gynecology & Obstetrics | Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsia [Pubmed:29569400] |
| Advances in Experimental?Medicine?and?Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028] |
| Taiwanese Journal of Obstetrics & Gynecology | HMGB1-RAGE signaling pathway in pPROM [Pubmed:29673663] |
| Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes [ISBN:978-951-51-4039-5] | |
| Journal?of?Pharmacological?Sciences | Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1 [Doi: 10.1016/j.jphs.2018.07.012] |
| Biomaterials Science | Delivery of 5'-Triphosphate RNA with Endosomolytic Nanoparticles Potently Activates RIG-I to Improve Cancer Immunotherapy [Doi: 10.1039/C8BM01064A?] |
| iris-AperTO [] | |
| Toxicological Sciences | Apoptosis resistance in fibroblasts precedes progressive scarring in pulmonary fibrosis and is partially mediated by Toll-like receptor 4 activation [Pubmed: 31020321] |
| Molecular Medicine | FBXW7 suppresses HMGB1-mediated innate immune signaling to attenuate hepatic inflammation and insulin resistance in a mouse model of nonalcoholic?… [Pubmed: 31215394] |
| international immunopharmacology | Dulaglutide mitigates inflammatory response in fibroblast-like synoviocytes [Pubmed: 31185450] |
| Food & Function | Network pharmacology oriented study reveals inflammatory state-dependent dietary supplement hepatotoxicity responses in normal and diseased rats [Pubmed: 31140472] |
| International Immunopharmacology | BET bromodomain inhibitor JQ1 promotes immunogenic cell death in tongue squamous cell carcinoma [Pubmed: 31600692] |
| Experimental and Therapeutic Medicine | High mobility group box protein 1 neutralization therapy in ovine bacteremia: Lessons learned from an ovine septic shock model incorporating intensive care support [Pubmed: 31602200] |
| Oxidative Medicine and Cellular Longevity | Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters?… [Pubmed: 31737175] |
| Pathobiology | Magnetic hyperthermia using self-controlled heating elements consisting of Fe-Al milling alloy induces cancer cell apoptosis while preserving skeletal muscle [Pubmed: 31722347] |
| Canadian Journal of Physiology and Pharmacology | Suppression of high-mobility group box 1 ameliorates xerostomia in a sjogren syndrome-triggered mouse model [Pubmed: 31935120] |
| Neurotoxicity Research | HMGB1 Mediates Paraquat-Induced Neuroinflammatory Responses via Activating RAGE Signaling Pathway [Pubmed: 31858421] |
| Journal of Clinical Medicine | Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109] |
| Journal of Immunology Research | Dendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In Vitro [Pubmed: 31886313] |
| urologic oncology | A novel diagnostic tool for the detection of bladder cancer: Measurement of urinary high mobility group box-1 [Pubmed: 32312640] |
| International Journal of Environmental Research and Public Health | Marathon Run-induced Changes in the Erythropoietin-Erythroferrone-Hepcidin Axis are Iron Dependent [Pubmed: 32316587] |
| VETERINARY MICROBIOLOGY | Upregulation of HMGB1 Secretion in Lungs of Pigs Infected by Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus [Pubmed: 33221069] |
| Lab Invest | Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis [Pubmed: 32709888] |
| American Journal of Translational Research | Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and … [Pubmed: 32509190] |
| STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … [Pubmed: 32406006] |
| Translational Stroke Research | Glycyrrhizin Prevents Hemorrhagic Transformation and Improves Neurological Outcome in Ischemic Stroke with Delayed Thrombolysis Through Targeting?… [] |
| stem cells international | Hypoxic Wharton's Jelly Stem Cell Conditioned Medium Induces Immunogenic Cell Death in Lymphoma Cells [] |
| Frontiers in Cell and Developmental Biology | Aberrant Expression of High Mobility Group Box Protein 1 in the Idiopathic Inflammatory Myopathies [] |
| Naja sumatrana venom cytotoxin, sumaCTX exhibits concentration-dependent cytotoxicity via caspase-activated mitochondrial-mediated apoptosis without?… [] | |
| Journal of Burn Care & Research | Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats [33313794] |
| Cell Death & Disease | DAMPs released by pyroptotic cells as major contributors and therapeutic targets for CAR-T-related toxicities [33504767] |
| Cells | Insights into P-Glycoprotein Inhibitors: New Inducers of Immunogenic Cell Death [32331368] |
| Infect Immun | A non-lethal murine flame burn model leads to a transient reduction in host defenses and enhanced susceptibility to lethal Pseudomonas aeruginosa infection [34152806] |
| Inhaled [D-Ala2]-Dynorphin 1-6 Prevents Hyperacetylation and Release of High Mobility Group Box 1 in a Mouse Model of Acute Lung Injury [34616852] | |
| Mol Cell Biochem | Autophagy-associated HMGB-1 as a novel potential circulating non-invasive diagnostic marker for detection of Urothelial Carcinoma of Bladder [34796446] |
| World J Gastroenterol | Calycosin attenuates severe acute pancreatitis-associated acute lung injury by curtailing high mobility group box 1-induced inflammation [34908806] |
| Endocr J | Clinical significance of high mobility group box 1/toll-like receptor 4 in obese diabetic patients [34657898] |
| Research in Veterinary Science | Choline or CDP-choline restores hypotension and improves myocardial and respiratory functions in dogs with experimentally–Induced endotoxic shock [34715589] |
| Sci Rep | Intracellular translocation of HMGB1 is important for Zika virus replication in Huh7 cells [Pubmed:35058496] |
| ORL J Otorhinolaryngol Relat Spec | High Mobility Group Box-1 Protein and Interleukin 33 Expression in Allergic Rhinitis [Pubmed:34979505] |
| J Exp Clin Cancer Res | SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma [Pubmed:35197103] |
| Am J Physiol Gastrointest Liver Physiol | Abnormal intestinal milieu in post-traumatic stress disorder is not impacted by treatment that improves symptoms [Pubmed:35638693] |
| Journal of the South African Veterinary Association | Circulating markers of endothelial activation in canine parvoviral enteritis [] |
| Reseach square | The antiviral action of resveratrol against Zika virus through the regulation of interferon responses by HMGB1 [] |
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