肌肌酸激酶(CKM)單克隆抗體
Monoclonal Antibody to Creatine Kinase, Muscle (CKM)
CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed
- 編號(hào)UMAA109Hu28
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C10
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPA109Hu01-肌肌酸激酶(CKM)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Oryctolagus cuniculus (Rabbit，兔)
- 應(yīng)用WB; ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 363 ¥ 846 ¥ 1209 ¥ 3023 ¥ 12090
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Mouse Anti-Human CKM Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Samples: Lane1: Mouse Heart lysate; Lane2: Mouse Skeletal muscle lysate; Primary Ab: 5μg/ml Mouse Anti-Human CKM Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Samples: Lane1: Rabbit Cerebrum lysate; Lane2: Rabbit Skeletal muscle lysate; Lane3: Rabbit Heart lysate;
Primary Ab: 0.5μg/ml Mouse Anti-Human CKM Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Human THP1 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human CKM Monoclonal Antibody (Catalog MAA109Hu28, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)CKM的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CKM。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA109Hu01 | 肌肌酸激酶(CKM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA109Hu01 | 肌肌酸激酶(CKM)多克隆抗體 | WB; IHC |
| ULAA109Hu71 | 肌肌酸激酶(CKM)多克隆抗體(生物素標(biāo)記) | WB |
| UMAA109Hu27 | 肌肌酸激酶(CKM)單克隆抗體 | WB |
| UMAA109Hu29 | 肌肌酸激酶(CKM)單克隆抗體 | WB |
| UMAA109Hu26 | 肌肌酸激酶(CKM)單克隆抗體 | ICC/IF |
| UMAA109Hu23 | 肌肌酸激酶(CKM)單克隆抗體 | WB; IHC |
| UMAA109Hu24 | 肌肌酸激酶(CKM)單克隆抗體 | WB; IHC |
| UMAA109Hu25 | 肌肌酸激酶(CKM)單克隆抗體 | WB; IHC |
| UMAA109Hu22 | 肌肌酸激酶(CKM)單克隆抗體 | WB; IHC |
| UMAA109Hu28 | 肌肌酸激酶(CKM)單克隆抗體 | WB; ICC/IF; FCM |
| UMAA109Hu21 | 肌肌酸激酶(CKM)單克隆抗體 | WB; IHC |
| UFAA109Hu08 | 抗肌肌酸激酶(CKM)單克隆抗體 | FCM |
| UFAA109Hu06 | 抗肌肌酸激酶(CKM)單克隆抗體 | FCM |
| UFAA109Hu04 | 抗肌肌酸激酶(CKM)單克隆抗體 | FCM |
| UFAA109Hu02 | 抗肌肌酸激酶(CKM)單克隆抗體 | FCM |
| USEA109Hu | 肌肌酸激酶(CKM)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA109Hu | 肌肌酸激酶(CKM)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA109Hu01 | 肌肌酸激酶(CKM)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Inflammation | Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery [PubMed: 22583529] |
| PLoS One | Extraocular Muscle Characteristics Related to Myasthenia Gravis Susceptibility [PubMed: PMC3568149] |
| Kidney Int. | Fructokinase activity mediates dehydration-induced renal injury [Pubmed: 24336030] |
| Methylxanthines and cardiotoxicity | Methylxanthine induced cardiotoxicity and its mechanisms: An experimental study [230b:34bf146150b42971d76dda5e8329bc5f2f0b.pdf] |
| Molecular Medicine Reports | Protective effects of N (2)?L?alanyl?L?glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473] |
| Toxicological?Sciences | Proteomic and Metabolomic Profiling Identify Plasma Biomarkers for Exposure to Ultra-low Levels of Carfentanil [Pubmed: 30321427] |
| Clinical & Translational Oncology | The effects of human sera conditioned by high-intensity exercise sessions and training on the tumorigenic potential of cancer cells [Pubmed: 32447643] |
