甲胎蛋白(AFP)單克隆抗體
Monoclonal Antibody to Alpha-Fetoprotein (AFP)
aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
- 編號(hào)UMAA153Hu21
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C6
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPA153Hu01-甲胎蛋白(AFP)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Rhesus monkey (Simian，恒河猴)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 306 ¥ 714 ¥ 1020 ¥ 2550 ¥ 10200
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 10μg/ml Mouse Anti-Human aFP Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
-
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human aFP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human aFP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 10μg/ml Mouse Anti-Human aFP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 10μg/ml Mouse Anti-Human aFP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 10μg/ml Mouse Anti-Human aFP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
-
Western Blot; Sample: Simian Serum Primary Ab: 6μg/ml Mouse Anti-Human aFP Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
-
Western Blot; Sample: Lane1: Human Serum; Lane2: Human Liver lysate; Lane3: Human Lung lysate
Primary Ab: 3μg/ml Mouse Anti-Human aFP Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Samples: Lane1: Human Serum; Lane2: Human Placenta lysate; Lane3: LO2 cell lysate;
Primary Ab: 0.1μg/ml Mouse Anti-Human aFP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
-
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)AFP的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別AFP。
用法
Western blottin:g: 0.5-6μg/mL;
Immunohistochemistry: 5-60μg/mL;
Immunocytochemistry: 5-60μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA153Hu02 | 甲胎蛋白(AFP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA153Hu01 | 甲胎蛋白(AFP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA153Hu02 | 甲胎蛋白(AFP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA153Hu01 | 甲胎蛋白(AFP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA153Hu01 | 甲胎蛋白(AFP)多克隆抗體 | WB; IHC |
| UPAA153Hu02 | 甲胎蛋白(AFP)多克隆抗體 | WB |
| ULAA153Hu71 | 甲胎蛋白(AFP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA153Hu81 | 甲胎蛋白(AFP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA153Hu21 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC |
| UMAA153Hu22 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC |
| UMAA153Hu26 | 甲胎蛋白(AFP)單克隆抗體 | WB |
| UMAA153Hu23 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; FCM |
| UMAA153Hu24 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC |
| UMAA153Hu25 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC |
| UMAA153Hu27 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC |
| UMAA153Hu29 | 甲胎蛋白(AFP)單克隆抗體 | WB |
| UFAA153Hu02 | 抗甲胎蛋白(AFP)單克隆抗體 | FCM |
| UFAA153Hu04 | 抗甲胎蛋白(AFP)單克隆抗體 | FCM |
| UFAA153Hu06 | 抗甲胎蛋白(AFP)單克隆抗體 | FCM |
| UFAA153Hu08 | 抗甲胎蛋白(AFP)單克隆抗體 | FCM |
| USEA153Hu | 甲胎蛋白(AFP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA153Hu | 甲胎蛋白(AFP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA153Hu | 甲胎蛋白(AFP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA153Hu01 | 甲胎蛋白(AFP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| BioFactors | Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model [PubMed: 20872761] |
| Food and Nutrition Sciences | Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. [Scirp: 5684] |
| Diabetes Research and Clinical Practice | Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats [ScienceDirect: S0168822711000520] |
| Life Sciences | Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver [ScienceDirect: S0024320511002700] |
| Annals of Hepatology | Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. [Hepatology: source] |
| Biomedicine & Aging Pathology | Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats [ScienceDirect: S2210522013000348] |
| European journal of pharmacology | Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme [Pubmed: 24530413] |
| Asian Pac J Cancer Prev. | Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. [Pubmed:25684529] |
| Journal of Analytical Atomic Spectrometry | Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry [Rsc:Source] |
| PLOS ONE | Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma [Pubmed:24927126] |
| BMC Med Imaging | Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma [PubMed: 25981587] |
| Tumour Biol | Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma [PubMed: 26643893] |
| Acta Veterinaria Brno | The concentrations of selected blood serum proteins in calves during the first three months of life [85:33] |
| Hepatobiliary Pancreat Dis Int | Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern [abstract:abstract4525.shtml] |
| Journal of Applied Pharmaceutical Science | Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats [2071_pdf.pdf] |
| Frontiers in Physiology? | Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats [v04n07a07a] |
| International Journal of?Clinical?and Experimental?Medicine | Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model [1345-1349] |
| Tumor Biology | Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway [pubmed:28651490] |
| Advances in Clinical and Experimental Medicine | Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles [ISSN 2451-2680] |
| Tumor biology | Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats [pubmed:28720064] |
| EBioMedicine | Lnc-PCDH9-13: 1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular Carcinoma [Pubmed:30045829] |
| Sensors and Actuators B: Chemical | One-pot synthesis of a highly selective carboxyl-functionalized superparamagnetic probes for detection of alpha-fetoprotein [10.1016:j.snb.2018.03.038] |
| Biomedicine & Pharmacotherapy | Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and [Pubmed:29669302] |
| Journal of Investigative Surgery | Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation [Pubmed:28107094] |
| Asian?Pacific?Journal of?Cancer?Prevention | Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats [Pubmed:29693337] |
| Antioxid Redox Signal | The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma [Pubmed:29687726] |
| Cancer Biology | Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer [] |
| Cancer Biology | Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine [] |
| Biomedicine & Pharmacotherapy | The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB [Pubmed: 30784932] |
| Asian Journal of Pharmacy and Pharmacology | Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma [] |
| American Journal of Transplantation | Circulating exosomal miR‐92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence [Pubmed: 31162867] |
| Environ Toxicol Pharmacol. | Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats [Pubmed: 31421311] |
| Hepatology Research | Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition [Pubmed: 31652385] |
| REDOX REPORT | Selective cytotoxic activity and protective effects of sodium ascorbate against hepatocellular carcinoma through its effect on oxidative stress and apoptosis in vivo and … [Pubmed: 32172678] |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440] |
| Nutr Cancer | Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation [Pubmed: 32718197] |
| Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles [] | |
| Microchemical Journal | Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS [] |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats [] | |
| Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo [] | |
| DEVELOPMENT | Blocking estrogen-induced AMH expression is crucial for normal follicle formation [33658225] |
| Cytokine | QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model [34564023] |
| Journal of Gastrointestinal Cancer | In Vivo Study of a Newly Synthesized Chromen-4-One Derivative as an Antitumor Agent Against HCC [34698995] |
留言咨詢
