皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Cortisol (Cor)
Hydrocortisone
- 編號UMEA462Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長3h
- 檢測范圍12.35-1,000ng/mL
- 靈敏度最小可檢測劑量小于等于4.85ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1541 ¥ 2202 ¥ 9909 ¥ 18717 ¥ 154140
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-102 | 94 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 78-99 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-97% | 89-97% | 83-99% | 84-91% |
| EDTA plasma(n=5) | 80-103% | 93-101% | 90-101% | 84-101% |
| heparin plasma(n=5) | 88-98% | 85-99% | 85-99% | 87-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL后，立即加入檢測溶液A25µL，
37°C孵育1小時；
3. 甩干，洗板3次；
4. 加檢測溶液B50µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分鐘；
7. 加終止液25µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經(jīng)洗滌去掉未結合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質(zhì)含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| UCPA462Ge11 | 皮質(zhì)醇(Cor)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA462Ge21 | 皮質(zhì)醇(Cor)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA462Ge01 | 皮質(zhì)醇(Cor)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA462Ge02 | 皮質(zhì)醇(Cor)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAA462Ge81 | 皮質(zhì)醇(Cor)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA462Ge71 | 皮質(zhì)醇(Cor)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA462Ge21 | 皮質(zhì)醇(Cor)單克隆抗體 | IHC |
| UMAA462Ge22 | 皮質(zhì)醇(Cor)單克隆抗體 | ELISA |
| UMEA462Ge | 皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA462Ge | 皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA462Ge | 皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA462Ge | 皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA462Ge | 皮質(zhì)醇(Cor)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA462Ge | 皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA462Ge11 | 皮質(zhì)醇(Cor)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| JBCD | Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental students [Index.Php: Jbcd] |
| International Journal of Immunopathology & Pharmacology | Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638] |
| IET Micro & Nano Letters | Surface plasmon resonance immunoassay for cortisol determination with a self-assembling denaturalised bovine serum albumin layer on surface plasmon resonance chip [xpls:7393900] |
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| AQUACULTURE RESEARCH | Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition [] |
| AQUACULTURE RESEARCH | Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense [] |
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| J Am Heart Assoc | Post?\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest [33960200] |
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