催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay)
Pitocin; Syntocinon
(注:單次混測多因子不超過8個指標 )
- 編號ULMB052Ge
- 物種Pan-species (General,通用) 相同的名稱,不同的物種。
- 實驗方法競爭抑制
- 反應時長1.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數 7指標數 6指標數 5指標數 4指標數 3指標數 2指標數1指標數
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特異性
本試劑盒用于檢測催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 79-101 | 82 |
EDTA plasma(n=5) | 85-99 | 90 |
heparin plasma(n=5) | 89-97 | 94 |
sodium citrate plasma(n=5) | 79-98 | 93 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內加入適量的催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-101% | 94-101% | 92-103% | 85-101% |
EDTA plasma(n=5) | 90-105% | 81-96% | 99-105% | 78-105% |
heparin plasma(n=5) | 85-99% | 82-103% | 95-102% | 78-98% |
sodium citrate plasma(n=5) | 94-102% | 83-95% | 80-93% | 85-103% |
穩(wěn)定性
經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠、檢測溶液A)標準品或樣本50μL及磁珠10μL,加檢測溶液A50μL,
37°C酶標板振蕩器孵育60分鐘;
3. 磁吸洗板3次;
4. 加檢測溶液B100μL,37°C振動孵育30分鐘;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋渦震蕩2分鐘后讀數。
實驗原理
將催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠、標記VEGFA,標記的催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合,然后將未結合物洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數。MFI值和樣品中的催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。
贈品
增值服務
相關產品
編號 | 適用物種:Pan-species (General,通用) | 應用(僅供研究使用,不用于臨床診斷!) |
UCPB052Ge11 | 催產素(OT)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
UCPB052Ge21 | 催產素(OT)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
UPAB052Ge01 | 催產素(OT)多克隆抗體 | ELISA\ |
UMEB052Ge | 催產素(OT)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
UHEB052Ge | 催產素(OT)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
UCEB052Ge | 催產素(OT)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
ULMB052Ge | 催產素(OT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
UKSB052Ge11 | 催產素(OT)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
J Psychiatr Res.? | Sex-specific associations between plasma oxytocin levels and schizotypal personality features in healthy individuals [Pubmed:24411593] |
Fertility and Sterility | Administration of atosiban in patients with endometriosis undergoing frozena??thawed embryo transfer: a prospective, randomized study [Pubmed:27143518] |
Progress in neuro-psychopharmacology & biological psychiatry | Increased oxytocin levels among abstinent heroin addicts: Association with aggressiveness, psychiatric symptoms and perceived childhood neglect. [pubmed:28093220] |
International Journal of Clinical and Experimental Medicine | Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic … [files:ijcem0031358.pdf] |
Fertility and Sterility | Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study [pubmed:27143518] |
BMC Med. | Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial. [pubmed:28209155] |
Psychiatry Research | Plasma oxytocin levels in major depressive and bipolar II disorders [pubmed:28865715] |
BMC Medicine | Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial [10.1186:s12916-017-0801-0] |
EUROPEAN?ADDICTION?RESEARCH | The Association between the Level of Plasma Oxytocin and Craving among Former Heroin Users [Pubmed:29902803] |
ASN Neuro . | Involvement of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 in Oxytocin Neuronal Activity in Lactating Rats With Pup Deprivation [Pubmed: 32962418] |
European Journal of Integrative Medicine | Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized?… [] |
J. Pers. Med | Predicting the Treatment Outcomes of Antidepressants Using a Deep Neural Network of Deep Learning in Drug-Na?ve Major Depressive Patients [] |