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白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Interleukin 8 (IL8) ,etc. by FLIA (Flow Luminescence Immunoassay)

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

（注：單次混測多因子不超過8個指標）

編號ULMA080Ga
物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實驗方法雙抗夾心
反應(yīng)時長3.5h
檢測范圍0.98-1000pg/mL
靈敏度最小可檢測劑量小于等于0.327 pg/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	88-96	93
EDTA plasma(n=5)	96-104	101
heparin plasma(n=5)	83-102	97

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	94-102%	87-96%	92-101%	90-97%
EDTA plasma(n=5)	78-99%	94-105%	83-101%	78-90%
heparin plasma(n=5)	85-98%	94-102%	95-103%	93-102%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

增值服務(wù)

實時熒光定量PCR實驗服務(wù)

參考文獻

雜志	參考文獻
Reproductive Biology and Endocrinology	Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow [Rbej: 14777827]
Acta Otolaryngol	Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa [PubMed: 19958244]
Journal of Clinical Periodontology	Experimental peri-implant mucositis at different implant surfaces. [Pubmed: 24521508]
Physiological Research	Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. [Pubmed: 2432969]
Rheumatology International	Serum levels of selected chemokines in systemic lupus erythematosus patients [Pubmed: 22461186]
J Med Life.	Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination [Pubmed:Pmc4197484]
J Clin Periodontol.	Experimental peri‐implant mucositis at different implant surfaces [Pubmed:24521508]
British Journal of Nutrition	Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers [Pubmed:24846452]
Theriogenology	Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis [Theriojournal:Source]
Anim Reprod Sci.	Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes [PubMed: 25851495]
Медицина и здравоохранение	ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕ [Article: N]
BMC Anesthesiology	Hyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioning [PubMed: 25943099]
J Breath Res	Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery [PubMed: 25719610]
ARTICLE	The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt [Profile: Mohmad_Mosaad]
Veterinary Immunology and Immunopathology	Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period [Pubmed:27090626]
Infection, Genetics and Evolution	Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression [Pubmed:27349993]
Asian Pacific Journal of Tropical Medicine	Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury [science:S1995764516300955]
Archives of Biological Sciences	Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu]
Journal of Agricultural Biotechnology	Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells [10610]
journal of molecular and cellular cardiology	Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. [pubmed:28088561]
Physiological Research	Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury. [pubmed:28006948]
PLOS?ONE	Mechanical?Ventilation?Alters?the?Development?of?Staphylococcus?aureus?Pneumonia?in?Rabbit. [pubmed:27391952]
The Journal of Comenius University in Bratislava	Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury [acm.2017.16.issue-3]
Journal of Physiology and Pharmacology	Effects?of?budesonide?on the?lung?functions,?inflammation?and?apoptosis?in a?saline-lavagemodel?of?acute?lung?injury. [pubmed:28195073]
中國藥物警戒	小劑量阿奇霉素對穩(wěn)定期老年慢性阻塞性肺疾病患者外周血內(nèi)TNF-α、IL-8、CRP水平及肺功能的影響 [Periodical_zgywjj201608004]
Veterinary Immunology and Immunopathology	Cytokine levels in colostrum and in foals' serum pre- and post-suckling [pubmed:28242000]
Journal of Cellular Physiology	Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057]
European Journal of Pharmacology	Cardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat study [pubmed:28684235]
PLoS One	Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilation [pubmed:29149185]
Archives of Oral Biology	The effect of restorative materials on cytokines in gingival crevicular fluid [pubmed:28992599]
	Experimentelle periimplant?re Mukositis an unterschiedlichen Implantatoberfl?chen [doi: 10.1111/jcpe.12240.]
Molecular?Medicine?Reports	CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB [pubmed:28627614]
ARTIFICIAL?CELLS?NANOMEDICINE AND BIOTECHNOLOGY?	Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis [pubmed:28608724]
Biological Rhythm Research	Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows [10.1080/09291016.2017.1319636]
Scientific Reports	Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665]
Journal of Ultrasound in Medicine	Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells. [pubmed:28600899]
Veterinary Immunology and Immunopathology	Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. [pubmed:28895861]
Biomedicine hub	Comparison of Polymyxin E and Polymyxin B as an Additive to Pulmonary Surfactant in Escherichia coli Pneumonia of Ventilated Neonatal Rabbits [10.1159/000475877]
Anticancer Research	Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab. [pubmed:28982871]
Chronic Respiratory Disease	Inflammatory biomarkers and radiologic measurements in never-smokers with COPD: A cross-sectional study from the CODA cohort. [pubmed:29117798]
Inflammopharmacology	Polar extract of protects cartilage homeostasis: possible mechanism of action [Pubmed:29313174]
Life?Sciences	Dexmedetomidine attenuates renal fibrosis via α2-adrenergic receptor-dependent inhibition of cellular senescence after renal ischemia/reperfusion [Pubmed:29729264]
Microbial Pathogenesis	Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos?… [Pubmed:29787791]
Lasers in Medical Science	Evaluation of the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears [Pubmed:30003425]
Medical Science Monitor	Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)?… [Pubmed:29804125]
Journal of Veterinary?Science	Rifaximin anti‐inflammatory activity on bovine endometrium primary cell cultures: a preliminary study [Pubmed:29984902]
Bioscience Reports	N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. [Doi: 10.1042/BSR20180858]
Acta?Medica?Mediterranea	Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung?… [Doi: 10.1515/acm-2016-0012]
Journal of?Dairy?Science	Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows [Pubmed: 17430930]
Biomedicine?&?Pharmacotherapy	Interleukin 8 targeted contrast echocardiography is effective to evaluate myocardial ischemia-reperfusion injury in the rabbits []
Emerging?Microbes?&?Infections	Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin [Pubmed: 30405111]
Journal of Investigative?Medicine	The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494]
International Journal of Chronic Obstructive Pulmonary Disease	Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD [Pubmed: 30655663]
Inflammopharmacology	Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard & []
Journal of Hematology &?Oncology	Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment?… [Pubmed: 30621731]
BioMed Research International	Effective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale Extracts []
Frontiers in Pharmacology	Naoxintong Capsule Inhibits the Development of Cardiovascular Pathological Changes in Bama Minipig Through Improving Gut Microbiota [Pubmed: 31632272]
international journal of molecular sciences	Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells [Pubmed: 31671764]
Scientific Reports	Corrosion Resistance of Graphene oxide/Silver Coatings on Ni–Ti alloy and Expression of IL-6 and IL-8 in Human Oral Fibroblasts [Pubmed: 32094428]
Journal of Receptors and Signal Transduction Research	Suppressor of ras val-2 promotes inflammation-mediated oxidative stress and cell apoptosis in cardiomyocytes through activating Mst1-mROS signaling pathway [Pubmed: 32065019]
Antibiotics	Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs [Pubmed: 31963653]
Physiol Res	Effect of Different Dosages of Dexamethasone Therapy on Lung Function and Inflammation in an Early Phase of Acute Respiratory Distress Syndrome Model [Pubmed: 31928043]
Respiratory Research	Synthetic surfactant with a recombinant surfactant protein C analogue improves lung function and attenuates inflammation in a model of acute respiratory?… [Pubmed: 31694668]
Journal of Pharmacy and Pharmacology	Tectorigenin inhibits inflammation and pulmonary fibrosis in allergic asthma model of ovalbumin‐sensitized guinea pigs [Pubmed: 32314371]
Inflammation	Cyanidin-3-O-Glucoside Attenuates Lipopolysaccharide-Induced Inflammation in Human Corneal Epithelial Cells by Inducing Let-7b-5p-Mediated HMGA2/PI3K/Akt?… [Pubmed: 32248330]
Sci Rep	NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han population [Pubmed: 33177541]
International Journal of Nanomedicine	Study on Adenovirus Infection in vitro with Nanoself-Assembling Peptide as Scaffolds for 3D Culture [Pubmed: 32922004]
Theriogenology	A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows [Pubmed: 32810791]
Cell Cycle	SIRT1 relieves Necrotizing Enterocolitis through inactivation of Hypoxia-inducible factor (HIF)-1a [Pubmed: 32657204]
Frontiers in Pharmacology	Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating Autophagy [Pubmed: 32508661]
Indian Journal of Animal Sciences	Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy?… []
Animals	Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period … []
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE	Possible Anti-inflammatory Effects of Mesenchymal Stem Cells Transplantation via Changes in CXCL8 Levels in Patients with Refractory Rheumatoid Arthritis []
	HMGB1 e citocinas inflamatórias em les?o pulmonar aguda experimental induzida em coelhos []
Bull Exp Biol Med	Inflammation Mediators Regulate the Microbiota Resistance to Adverse Factors [33222085]
physiological reports	Impact of synthetic surfactant CHF5633 with SP?\B and SP?\C analogues on lung function and inflammation in rabbit model of acute respiratory distress syndrome [33403805]
J Med Food	Cough Inhibition Activity of Schisandra chinensis in Guinea Pigs [33861937]
Sci Rep	Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population [33850223]
Sci Rep	Corrosion Resistance of Graphene oxide/Silver Coatings on Ni¨CTi alloy and Expression of IL-6 and IL-8 in Human O... [32094428]
Poultry Science	Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine??- [33518110]
Bioactive Materials	Construction of heparin-based hydrogel incorporated with Cu5. 4O ultrasmall nanozymes for wound healing and inflammation inhibition [33778192]
animals	Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic??- [34066204]
Sci Rep	Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients [34376749]
Pharmaceutics	Nitric-Oxide-Releasing Dexamethasone Derivative NCX-1005 Improves Lung Function and Attenuates Inflammation in Experimental Lavage-Induced ARDS [34959373]
Biomedicines	Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes [34829910]
Anesthesiology	Adverse Mechanical Ventilation and Pneumococcal Pneumonia Induce Immune and Mitochondrial Dysfunctions Mitigated by Mesenchymal Stem Cells in Rabbits [Pubmed:34965287]
J Clin Med	Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot Study [Pubmed:35566605]
Microb Pathog	Cell damage and neutrophils promote the infection of Mycoplasma pneumoniae and inflammatory response [Pubmed:35724831]
ResearchSquare	LncRNA KCNQ10T1 shuttled by bone marrow mesenchymal stem cells-derived exosome inhibits sepsis via regulation of miR-154-3p/RNF19A axis []
	Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS []
Intensive Care Research	Evaluating the Roles of sCD14 and sCD14-ST in Diagnosing COPD and Predicting an Acute Exacerbation of COPD []

留言咨詢

編號	適用物種：Chicken (Gallus，雞)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA080Ga01	白介素8(IL8)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPA080Ga01	白介素8(IL8)活性蛋白	Cell?culture;?Activity?Assays.
UPAA080Ga01	白介素8(IL8)多克隆抗體	WB; IHC; ICC; IP.
ULAA080Ga71	白介素8(IL8)多克隆抗體(生物素標記)	WB; IHC; ICC.
ULAA080Ga81	白介素8(IL8)多克隆抗體(異硫氰酸熒光素標記)	WB; IHC; ICC; IF.
UMAA080Ga22	白介素8(IL8)單克隆抗體	WB; IHC; ICC; IP.
UMAA080Ga21	白介素8(IL8)單克隆抗體	ICC/IF
UMAA080Ga23	白介素8(IL8)單克隆抗體	ICC/IF
USEA080Ga	白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
UIEA080Ga	白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA080Ga	白介素8(IL8)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UPSA080Ga01	白介素8(IL8)抗體對	ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
UKSA080Ga01	白介素8(IL8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".