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白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Interleukin 5 (IL5) ,etc. by FLIA (Flow Luminescence Immunoassay)

E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor

（注：單次混測多因子不超過8個指標）

編號ULMA078Mu
物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實驗方法雙抗夾心
反應時長3.5h
檢測范圍0.49-500pg/mL
靈敏度最小可檢測劑量小于等于0.163 pg/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	88-98	91
EDTA plasma(n=5)	78-101	86
heparin plasma(n=5)	82-92	88

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	98-105%	94-101%	84-102%	88-102%
EDTA plasma(n=5)	95-105%	78-91%	84-101%	98-105%
heparin plasma(n=5)	82-97%	80-93%	98-105%	88-95%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

增值服務

實時熒光定量PCR實驗服務

參考文獻

雜志	參考文獻
Critical Care Medicine	Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056]
veterinary research forum	Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ]
Molecular Immunology	Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-??B in murine model of asthma [Pubmed:27289030]
Advances in Experimental Medicine and Biology	Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Cavia (Guinea pig )inea Pigs [Pubmed:27130219]
Sci Rep.	Interleukin-31 expression and relation to disease severity in human asthma [pmc:PMC4783779]
Molecular?Immunology	Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma [pubmed:27289030]
Gut Pathog.	Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847]
Asian Pacific Society of Respirology	Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397]
Acta?Pharmacologica Sinica	Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi') [Pubmed: 19363516]
International Journal of Peptide Research and Therapeutics	ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma []
Journal of?Pineal?Research	Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267]
Analytica Chimica Acta	An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis []
American Journal of Translational Research	Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193]
bioscience reports	Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767]
Biomedicine & Pharmacotherapy	Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice [Pubmed: 32521453]
Journal of Traditional Chinese Medical Sciences	Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice []
	Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota []
Annals of Tropical Medicine & Public Health	Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq []
PLoS One	Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928]
Mol Immunol	MiR-142-5p directly targets cyclin-dependent kinase 5-mediated upregulation of the inflammatory process in acquired middle ear cholesteatoma [34875451]
Mljekarstvo	Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows []

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編號	適用物種：Mus musculus (Mouse，小鼠)	應用(僅供研究使用，不用于臨床診斷！)
URPA078Mu02	白介素5(IL5)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA078Mu01	白介素5(IL5)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPA078Mu61	白介素5(IL5)活性蛋白	Cell?culture;?Activity?Assays.
UEPA078Mu61	白介素5(IL5)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA078Mu02	白介素5(IL5)多克隆抗體	WB; IHC; ICC; IP.
UPAA078Mu01	白介素5(IL5)多克隆抗體	WB; IHC; ICC; IP.
UMEA078Mu	白介素5(IL5)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
USEA078Mu	白介素5(IL5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA078Mu	白介素5(IL5)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.