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白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Interleukin 5 (IL5) ,etc. by FLIA (Flow Luminescence Immunoassay)

E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor

（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）

編號(hào)ULMA078Eq
物種Equus caballus; Equine (Horse，馬) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實(shí)驗(yàn)方法雙抗夾心
反應(yīng)時(shí)長(zhǎng)3.5h
檢測(cè)范圍0.98-1000pg/mL
靈敏度最小可檢測(cè)劑量小于等于0.327 pg/mL.
樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	91-99	96
EDTA plasma(n=5)	97-104	101
heparin plasma(n=5)	90-103	95

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	89-98%	81-96%	90-101%	78-99%
EDTA plasma(n=5)	80-92%	80-96%	87-102%	84-91%
heparin plasma(n=5)	90-98%	78-91%	82-93%	97-105%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

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增值服務(wù)

實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Critical Care Medicine	Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056]
veterinary research forum	Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ]
Molecular Immunology	Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-??B in murine model of asthma [Pubmed:27289030]
Advances in Experimental Medicine and Biology	Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Cavia (Guinea pig )inea Pigs [Pubmed:27130219]
Sci Rep.	Interleukin-31 expression and relation to disease severity in human asthma [pmc:PMC4783779]
Molecular?Immunology	Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma [pubmed:27289030]
Gut Pathog.	Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847]
Asian Pacific Society of Respirology	Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397]
Acta?Pharmacologica Sinica	Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi') [Pubmed: 19363516]
International Journal of Peptide Research and Therapeutics	ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma []
Journal of?Pineal?Research	Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267]
Analytica Chimica Acta	An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis []
American Journal of Translational Research	Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193]
bioscience reports	Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767]
Biomedicine & Pharmacotherapy	Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice [Pubmed: 32521453]
Journal of Traditional Chinese Medical Sciences	Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice []
	Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota []
Annals of Tropical Medicine & Public Health	Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq []
PLoS One	Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928]
Mol Immunol	MiR-142-5p directly targets cyclin-dependent kinase 5-mediated upregulation of the inflammatory process in acquired middle ear cholesteatoma [34875451]
Mljekarstvo	Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows []

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編號(hào)	適用物種：Equus caballus; Equine (Horse，馬)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA078Eq01	白介素5(IL5)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA078Eq01	白介素5(IL5)多克隆抗體	WB; IHC; ICC; IP.
UMAA078Eq21	白介素5(IL5)單克隆抗體	WB; IHC; ICC; IP.
USEA078Eq	白介素5(IL5)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA078Eq	白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.