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免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Immunoglobulin M (IgM) ,etc. by FLIA (Flow Luminescence Immunoassay)

IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

（注：單次混測多因子不超過8個指標(biāo) ）

編號ULMA543Bo
物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實驗方法雙抗夾心
反應(yīng)時長3.5h
檢測范圍0.1-100ng/mL
靈敏度最小可檢測劑量小于等于0.033 ng/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	80-103	79
EDTA plasma(n=5)	88-98	93
heparin plasma(n=5)	81-88	84
sodium citrate plasma(n=5)	97-105	101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	87-97%	80-90%	96-105%	94-101%
EDTA plasma(n=5)	88-104%	96-105%	95-103%	78-92%
heparin plasma(n=5)	98-105%	87-103%	84-102%	88-95%
sodium citrate plasma(n=5)	82-101%	85-92%	96-104%	89-105%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

增值服務(wù)

實時熒光定量PCR實驗服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Poultry Science	Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ]
Clin Vaccine Immunol.	Speci?c Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice [Pubmed:24429068]
Asian J Pharm Clin Res	INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790]
Food and Agricultural Immunology	Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers [10.108:09540105.2017.1311308 ]
International Journal of?Clinical?and Experimental?Medicine	Detection of streptococcus pyogenes antibodies in acute idiopathic urticaria [ISSN:1940-5901/IJCEM0053690]
Food and Agricultural Immunology	Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers [10.1080:09540105.2018.1457013]
SAGE?Open?Medicine	Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes [Pubmed:29623201]
International Journal of Biological Macromolecules	Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens [Pubmed: 32224178]
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES	Physicochemical characterization and immunomodulatory activity of sulfated Chinese yam polysaccharide [Pubmed: 33010421]
BIOLOGICAL TRACE ELEMENT RESEARCH	Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225]
	IMMUNOSTIMULATORY EFFECT OF MORINGA OLEIFERA LEAVES EXTRACT ON CYCLOPHOSPHAMIDE-INDUCED IMMUNOSUPPRESSION IN MICE []
Journal of Functional Foods	Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts []
Italian Journal of Animal Science	Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the?… []
Regenerative Biomaterials	A comparative study of autogenous, allograft and artificial bone substitutes on bone regeneration and immunotoxicity in rat femur defect model []
博士論文	Women Scientist Scientist Mentor []
Life Sci	Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats [33577848]
Mol Med Rep	Baicalin relieves Mycoplasma pneumoniae infection?induced lung injury through regulating microRNA?221 to inhibit the TLR4/NF?\|êB signaling pathway [34109422]
Environ Pollut	Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073]
British Journal of Pharmacology	The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus []
Journal of Herbs, Spices & Medicinal Plants	Immunostimulatory Effect of Moringa oleifera Extracts on Cyclophosphamide-induced Immunosuppressed Mice []
Microbiology Spectrum	Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows [34494854]
Food Research International	Sulfated modification enhances the immunomodulatory effect of Cyclocarya paliurus polysaccharide on cyclophosphamide-induced immunosuppressed mice through?… [34865774]
Food Funct	Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093]

留言咨詢

編號	適用物種：Bos taurus; Bovine (Cattle，牛)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA543Bo01	免疫球蛋白M(IgM)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA543Bo01	免疫球蛋白M(IgM)多克隆抗體	WB
ULAA543Bo71	免疫球蛋白M(IgM)多克隆抗體(生物素標(biāo)記)	WB
UMAA543Bo21	免疫球蛋白M(IgM)單克隆抗體	WB; IHC; ICC; IP.
USEA543Bo	免疫球蛋白M(IgM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
UMEA543Bo	免疫球蛋白M(IgM)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA543Bo	免疫球蛋白M(IgM)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA543Bo01	免疫球蛋白M(IgM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".