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促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Gonadotropin Releasing Hormone (GnRH) ,etc. by FLIA (Flow Luminescence Immunoassay)

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

（注：單次混測多因子不超過8個指標(biāo) ）

編號ULMA843Fi
物種Danio rerio (Zebrafish，斑馬魚) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實驗方法競爭抑制
反應(yīng)時長1.5h
檢測范圍0.98-1000pg/mL
靈敏度最小可檢測劑量小于等于0.327 pg/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	83-101	89
EDTA plasma(n=5)	79-105	82
heparin plasma(n=5)	91-105	101
sodium citrate plasma(n=5)	98-105	102

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	82-101%	88-95%	78-91%	88-95%
EDTA plasma(n=5)	92-99%	95-104%	86-102%	86-104%
heparin plasma(n=5)	80-103%	80-94%	96-103%	83-104%
sodium citrate plasma(n=5)	96-104%	93-101%	90-99%	86-93%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。

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實時熒光定量PCR實驗服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Mol Pharmacol.	Maternal exposure to dioxin imprints sexual immaturity of the pups through fixing the status of the reduced expression of hypothalamic gonadotropin-releasing … [Pubmed:24132183]
Journal of Sports Science & Medicine	Heavy Resistance Training and Supplementation With the Alleged Testosterone Booster Nmda has No Effect on Body Composition, Muscle Performance, and Serum Hormones Associated With the Hypothalamo-Pituitary-Gonadal Axis in Resistance-Trained Males [Pubmed:24570624]
Environmental Research	Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141]
Toxicology Letters	Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392]
Toxicol Sci	Chronic exposure of female mice to an environmental level of perfluorooctane sulfonate suppresses estrogen synthesis through reduced histone H3K14 acetylation of the StAR promoter leading to deficits in follicular development and ovulation [PubMed: 26358002]
Int J Mol Sci.	Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice [Pubmed:26771609]
Oral Presentations	OP0019-HPR Effectiveness of A Progressive Resistance Strength Program on Hand Osteoarthritis: A Randomised Controlled Trial [60.1.abstract]
Oral Presentations	OP0021-HPR Sleep Disorders in Patients with Rheumatoid Arthritis and Relationship with Disease Activity [60.3.abstract]
Toxicological?Sciences	Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384]
Chronobiology of the immune reponse	OP0020?The Association of Gonadotropin-Releasing Hormone and Cytokines in Rheumatoid Arthritis [content:75]
Environmental Science & Technology	Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis [pubmed:28731686]
Toxicological?Sciences	Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci]
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BioMed Research International	Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
JOURNAL OF ETHNOPHARMACOLOGY	Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655]
J Virol	Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391]

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編號	適用物種：Danio rerio (Zebrafish，斑馬魚)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA843Fi01	促性腺激素釋放激素(GnRH)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA843Fi01	促性腺激素釋放激素(GnRH)多克隆抗體	WB; IHC; ICC; IP.
UCEA843Fi	促性腺激素釋放激素(GnRH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA843Fi	促性腺激素釋放激素(GnRH)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.