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鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay)

（注：單次混測多因子不超過8個指標）

編號ULMA518Ra
物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實驗方法雙抗夾心
反應(yīng)時長3.5h
檢測范圍0.2-200ng/mL
靈敏度最小可檢測劑量小于等于0.067 ng/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	94-101	97
EDTA plasma(n=5)	97-105	102
heparin plasma(n=5)	78-101	89

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	98-105%	80-102%	87-105%	88-98%
EDTA plasma(n=5)	89-98%	95-104%	78-105%	96-103%
heparin plasma(n=5)	93-102%	91-98%	87-97%	84-92%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

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參考文獻

雜志	參考文獻
Molecular Endocrinology	PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation. [Pubmed: 23438894]
experimental oncology	Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. [PubMed: 25804229]
J Cancer Sci Ther	Host H68D Genotype Affects Tumor Growth in Mouse Melanoma [Open-Access: 1948-5956-1000353]
Food Funct	The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699]
Physiological research	The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743]
Expert?Review?of Hematology	4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. [pubmed:27615544]
Oxidative Medicine and Cellular Longevity	Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463]
European Journal of Pharmaceutical Sciences	sucrosomial? iron absorption studied by in vitro and ex-vivo models [pubmed:29055735]
Journal of Food Science and Technology	In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. [pubmed:29051659]
International Journal of Cuban Health & Medicine	Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women [10.1590/MEDICC.2017.1902030004?]
Oman Medical Journal	The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599]
Food Bioscience	Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system [10.1016/j.fbio.2017.10.001]
Nutrients.	Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats [pubmed:28629118]
Food?Chemistry	Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells [Pubmed:29622185]
Animal Feed Science and Technology	Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet [10.1016:j.anifeedsci.2018.01.012]
American Journal of Hematology	Iron homeostasis in pregnancy and spontaneous abortion [Pubmed: 30394565]
The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria	Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria []
Haematologica	New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208]
博士論文	PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE []
	Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic []
Cell Research	Transferrin plays a central role in coagulation balance by interacting with clotting factors [Pubmed: 31811276]
international journal of biological macromolecules	Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications [Pubmed: 31904465]
journal of the american college of nutrition	Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation [Pubmed: 32255407]
LWT-FOOD SCIENCE AND TECHNOLOGY	Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability []
J Mol Cell Cardiol	Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233]
European Journal of Pharmacology	The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 [Pubmed:35700835]

留言咨詢

編號	適用物種：Rattus norvegicus (Rat，大鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
UPAA518Ra01	鐵蛋白(FE)多克隆抗體	WB; IHC; ICC; IP.
UWEA518Ra	鐵蛋白(FE)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)	Enzyme-linked immunosorbent assay for Antigen Detection.
USEA518Ra	鐵蛋白(FE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA518Ra	鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.