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紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Erythropoietin (EPO) ,etc. by FLIA (Flow Luminescence Immunoassay)

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

（注：單次混測多因子不超過8個指標）

編號ULMA028Ca
物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實驗方法雙抗夾心
反應時長3.5h
檢測范圍1.95-2000pg/mL
靈敏度最小可檢測劑量小于等于0.65 pg/mL.
樣本類型Serum, plasma and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	78-89	85
EDTA plasma(n=5)	79-92	85
heparin plasma(n=5)	82-95	86

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	78-93%	95-104%	83-101%	84-97%
EDTA plasma(n=5)	91-101%	79-93%	97-105%	95-103%
heparin plasma(n=5)	86-95%	98-105%	94-102%	78-104%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

增值服務

實時熒光定量PCR實驗服務

參考文獻

雜志	參考文獻
Endocrinology	Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring [Endo: source]
Journal of Ethnopharmacology	Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression [PubMed: 21333724]
PLOS ONE	CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source]
The Journal of Clinical Endocrinology & Metabolism	Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation [PubMed: 22948762]
Experimental & Molecular Medicine	Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression [PubMed: PMC3509184]
Biochem Biophys Res Commun.	Reevaluation of erythropoietin production by the nephron [Pubmed:24832733]
Nutrition.	Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. [Pubmed:2528040]
J Appl Physiol (1985).	Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness [Pubmed:25324511]
Expert Rev Proteomics	Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone [PubMed: 26496240]
Oncotarget	KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin [pubmed:26575329]
international journal of molecular sciences	Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. [pubmed:28216608]
Veterinary Clinical Pathology	Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease [pubmed:28186645]
IOS Press Content Library	An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease]
Oxidative Medicine and Cellular Longevity	Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463]
PLoS?Neglected?Tropical?Diseases	Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis [pubmed:28489854]
Neurochemistry International	Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia [S0197018617304783]
Pesquisa Veterinária Brasileira	Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease [10.1590/s0100-736x2017000600011?]
Medical Journal Armed Forces India	Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003]
Neurochemistry International	Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on?… [Pubmed:29180303]
Clinical and Experimental?Medicine	Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502]
Bulletin?of Experimental?Biology?and?Medicine	Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia [Pubmed:29308565]
Bulletin?of Experimental?Biology?and?Medicine	Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in?… [Pubmed: 30353332]
FASEB?JOURNAL	High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans []
Journal of Functional Foods	Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats []
博士論文	PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE []
Nutrition	Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind?… [Pubmed: 31743809]
BRITISH JOURNAL OF HAEMATOLOGY	Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans [Pubmed: 32866306]
JCI Insight.	Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes [Pubmed: 32759495]
Blood Cells, Molecules, and Diseases	EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness [Pubmed: 32470757]
Int J Endocrinol	miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia [34367277]
J Mater Sci Mater Med	Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules [Pubmed:35072831]
	Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells []

留言咨詢

編號	適用物種：Canis familiaris; Canine (Dog，犬)	應用(僅供研究使用，不用于臨床診斷！)
URPA028Ca01	紅細胞生成素(EPO)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPA028Ca61	紅細胞生成素(EPO)活性蛋白	Cell?culture;?Activity?Assays.
UEPA028Ca61	紅細胞生成素(EPO)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA028Ca01	紅細胞生成素(EPO)多克隆抗體	WB
UMAA028Ca21	紅細胞生成素(EPO)單克隆抗體	WB; IHC; ICC; IP.
USEA028Ca	紅細胞生成素(EPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA028Ca	紅細胞生成素(EPO)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.