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血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Angiotensin I Converting Enzyme (ACE) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

（注：單次混測多因子不超過8個指標）

編號ULMA004Ra
物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
實驗方法雙抗夾心
反應(yīng)時長3.5h
檢測范圍0.02-20ng/mL
靈敏度最小可檢測劑量小于等于0.007 ng/mL.
樣本類型Serum, plasma, cerebrospinal fluid and other biological fluids
下載英文說明書中文說明書
規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	78-96	82
EDTA plasma(n=5)	82-99	95
heparin plasma(n=5)	85-99	93

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	89-97%	81-105%	94-104%	94-102%
EDTA plasma(n=5)	78-104%	94-102%	83-96%	78-97%
heparin plasma(n=5)	90-98%	79-105%	98-105%	92-105%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

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參考文獻

雜志	參考文獻
Lipids in Health and disease	Association of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oil [PubMed: PMC2914008]
Malaysian Journal of Medical Sciences	Effect of Repeatedly Heated Palm Olein on Blood Pressure–Regulating Enzymes Activity and Lipid Peroxidation in Rats [Usm: MJMS19120]
Journal of Cancer Therapy	Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source]
The American journal of clinical nutrition	Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial. [Pubmed: 23553151]
Journal of Vascular Surgery	Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606]
Reprod Toxicol.?	ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467]
Chemical Research in Toxicology	Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
Chem Biol Interact	Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling [PubMed: 26079204]
Biomed Res Int	Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress [PubMed: 26640794]
Clinics (Sao Paulo)	Mechanisms [PubMed: 26602523]
Chemical Research in Toxicology	Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Intensive Care Medicine Experimental	Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3]
Evid Based Complement Alternat Med.	Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure Implications [Pubmed:27293465]
Вавиловский журнал генетики и селекции	Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией [30_Dubinina.pdf]
Animal Biology	The effect of heat stress on bull sperm quality and related HSPs expression [content:journals]
biomedicine & pharmacotherapy	Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636]
Biomedicine & Pharmacotherapy	Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester [pubmed:28992471]
Journal of Functional Foods	Antihypertensive effects of?Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033]
Molecules	Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet [Pubmed:29614743]
Journal of?Surgical?Research	Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841]
Intensive Care Medicine	Angiotensin-converting enzymes in acute respiratory distress syndrome []
International Journal of Biological Sciences	Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction Through AA Metabolism Modulated CYPs Pathway []
Medicine	Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
American Journal of Respiratory and Critical Care Medicine	Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511]
Int Immunopharmacol	Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro [Pubmed: 32388215]
Food Bioscience	Djulis (Chenopodium formosanum) and its bioactive compounds affect vasodilation, angiotensin converting enzyme activity, and hypertension []
Journal of Extracellular Vesicles	MiR155-5p in adventitial fibroblasts-derived extracellular vesicles inhibits vascular smooth muscle cell proliferation via suppressing angiotensin-converting enzyme?… []
Biotechnology and Pharmacy	Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats []
	Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?… []

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編號	適用物種：Rattus norvegicus (Rat，大鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA004Ra01	血管緊張素轉(zhuǎn)化酶(ACE)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA004Ra01	血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體	WB; IHC; ICC; IP.
UMAA004Ra21	血管緊張素轉(zhuǎn)化酶(ACE)單克隆抗體	WB; IHC; ICC; IP.
USEA004Ra	血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA004Ra	血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.