低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
- 編號USEA798Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.060ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測低氧誘導因子1α(HIF1a)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的低氧誘導因子1α(HIF1a)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 94-103 | 101 |
| heparin plasma(n=5) | 93-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的低氧誘導因子1α(HIF1a)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中低氧誘導因子1α(HIF1a)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-101% | 89-97% | 88-102% | 79-94% |
| EDTA plasma(n=5) | 78-101% | 80-103% | 88-97% | 91-98% |
| heparin plasma(n=5) | 80-92% | 96-105% | 82-102% | 92-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將低氧誘導因子1α(HIF1a)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的低氧誘導因子1α(HIF1a)與連接于固相載體上的抗體結合，然后加入生物素化的低氧誘導因子1α(HIF1a)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的低氧誘導因子1α(HIF1a)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA798Ra01 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA798Ra02 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA798Ra01 | 低氧誘導因子1α(HIF1a)多克隆抗體 | WB; IHC |
| UPAA798Ra02 | 低氧誘導因子1α(HIF1a)多克隆抗體 | IHC |
| ULAA798Ra71 | 低氧誘導因子1α(HIF1a)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA798Ra | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA798Ra | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA798Ra | 低氧誘導因子1α(HIF1a)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA798Ra | 低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Cancer Chemotherapy and Pharmacology | Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells. [PubMed: 19466611] |
| European Journal of Pharmacology | Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibition [ScienceDirect: S0014299909011352] |
| PLoS ONE | Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor Prognosis [PubMed: PMC3781153] |
| International Journal of Molecular Medicine | Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic rats [Pubmed: 24002353] |
| Canadian Journal of Physiology and Pharmacology | Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemia [Pubmed: 24289078] |
| US8569372 B2 | Method for preventing or treating a disease, disorder or condition induced by retina ischemia [US8569372] |
| J Surg Res. | Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. [Pubmed: 23816242] |
| PLoS One. | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source] |
| Journal of Physiological Anthropology | Telomeres are elongated in rats exposed to moderate altitude [Pubmed:Pmc4088304] |
| Molecular Medicine Reports | Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. [Pubmed:25384583] |
| International Journal of Pharmaceutics | The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF [Pubmed:25445537] |
| International Journal of Molecular Medicine | Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats [Pubmed:25524396] |
| International Journal of Developmental Neuroscience | Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth [PubMed: 25889224] |
| Eur Surg Res | Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys [PubMed: 25341590] |
| Sci Rep | Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system [PubMed: 25905434] |
| Evidence-Based Complementary and Alternative Medicine | Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae [pmc:PMC4794592] |
| Biol Trace Elem Res. | Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α [Pubmed:27121973] |
| Раны и раневые инфекции. | Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией [91] |
| Biological?Trace?Element?Research | Roles?of?Copper?in?Hepatocarcinogenesis?via?the?Activation?of?Hypoxia-Inducible?Factor-1α. [pubmed:27121973] |
| Experimental and Therapeutic Medicine | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis [etm:13] |
| Clinical Trials in Degenerative Diseases | Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975] |
| Annals of Clinical & Laboratory Science | R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme [pubmed:28667042] |
| Archives of Gynecology and Obstetrics | Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? [10.1007/s00404-017-4461-2] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee [pubmed:28123469] |
| Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003] |
| Journal of Physiological Anthropology | Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia [Pubmed:29454386] |
| Bulletin?of Experimental?Biology?and?Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia [Pubmed:29308565] |
| Microchimica?Acta | Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric test based on click-mediated growth of gold nanoparticles [Pubmed: 30209641] |
| Bulletin?of Experimental?Biology?and?Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in?… [Pubmed: 30353332] |
| Translational?Oncology | OKN-007 Increases temozolomide (TMZ) Sensitivity and Suppresses TMZ-Resistant Glioblastoma (GBM) Tumor Growth [Pubmed: 30468988] |
| West Indian Medical Journal | Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia. [] |
| Bulletin of Experimental Biology and Medicine | Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar Rats [Pubmed: 30903510] |
| The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency [] | |
| scandinavian journal of immunology | Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathway [Pubmed: 31055848] |
| JOURNAL OF PERIODONTOLOGY | A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A?… [Pubmed: 31020669] |
| Tissue Eng Part A | Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation [Pubmed: 31238789] |
| ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ [] | |
| molecular and cellular biochemistry | Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia [Pubmed: 31420792] |
| Molecules | Regulation of MicroRNA-155 and Its Related Genes Expression by Inositol Hexaphosphate in Colon Cancer Cells [Pubmed: 31744065] |
| Stem Cell Res Ther | Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus [Pubmed: 32894200] |
| J Cell Physiol | PLCε regulates metabolism and metastasis signaling via HIF‐1α/MEK/ERK pathway in prostate cancer [Pubmed: 32383180] |
| Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза [] | |
| Food Funct | Prenatal vitamin D supplementation reduces blood pressure and improves placental angiogenesis in an animal model of preeclampsia [33237074] |
| Eur J Pharmacol | Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ?- [33705802] |
| EXPERIMENTAL EYE RESEARCH | Astaxanthin mediated regulation of VEGF through HIF1|á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model [33789142] |
| scientific reports | Serum hypoxia-inducible factor-1|á and uterine artery Doppler ultrasound during the first trimester for prediction of preeclampsia [33758274] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
留言咨詢
