Ⅰ型膠原交聯(lián)羧基端肽(CTXI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI)
CTX-I; CTX1;
- 編號UCEA665Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍123.5-10,000pg/mL
- 靈敏度最小可檢測劑量小于等于50.3pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測Ⅰ型膠原交聯(lián)羧基端肽(CTXI)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅰ型膠原交聯(lián)羧基端肽(CTXI)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-101 | 84 |
| EDTA plasma(n=5) | 97-105 | 101 |
| heparin plasma(n=5) | 98-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅰ型膠原交聯(lián)羧基端肽(CTXI)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅰ型膠原交聯(lián)羧基端肽(CTXI)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-102% | 79-99% | 89-101% | 88-104% |
| EDTA plasma(n=5) | 94-101% | 92-101% | 88-103% | 86-93% |
| heparin plasma(n=5) | 80-99% | 88-102% | 89-98% | 95-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將Ⅰ型膠原交聯(lián)羧基端肽(CTXI)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCEA665Ra | Ⅰ型膠原交聯(lián)羧基端肽(CTXI)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA665Ra | Ⅰ型膠原交聯(lián)羧基端肽(CTXI)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA665Ra11 | Ⅰ型膠原交聯(lián)羧基端肽(CTXI)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| journal of bone and mineral research | Atp6v0d2 Is an Essential Component of the Osteoclast-Specific Proton Pump That Mediates Extracellular Acidification in Bone Resorption [PubMed: PMC2672205] |
| Bone | Borage and fish oils lifelong supplementation decreases inflammation and improves bone health in a murine model of senile osteoporosis [PubMed: 21664309] |
| Trials | Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. [Trials: 1745-6215] |
| Journal of Biological Chemistry | TRAF family member-associated NF-κB activator (TANK) is a negative regulator of osteoclastogenesis and bone formation [PubMed: 22773835] |
| The Journal of Clinical Endocrinology & Metabolism | A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular Risk [PubMed: PMC3462931] |
| Endocrinology | Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain [Endocrine: Source] |
| Am J Physiol Endocrinol Metab. | DPP IV inhibitor treatment attenuates bone loss and improves mechanical bone strength in male diabetic rats [Pubmed:25053403] |
| The American Journal of Cardiology | Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease [Pubmed:25073565] |
| Rheumatology (Oxford). | Bone resorption correlates with the frequency of CD5+ B cells in the blood of patients with rheumatoid arthritis [Pubmed:25193807] |
| XPO1/CRM1-Selective Inhibitors of Nuclear Export (SINE) reduce tumor spreading and improve overall survival in preclinical models of prostate cancer (PCa) [Pubmed:25284315] | |
| Rheumatology International | The presence of FCGR2B promoter or transmembrane region variant alleles leads to reduced serum IL-6 levels in rheumatoid arthritis [Pubmed:25630523] |
| The Journal of Biological Chemistry | 5-azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival [Pubmed:25691576] |
| Scandinavian Journal of Clinical and Laboratory Investigation | Osteocartilaginous metabolic markers change over a 3-week stage race in pro-cyclists [PubMed: 26174975] |
| AIDS | Effects of renal tubular dysfunction on bone in tenofovir-exposed HIV-positive patients [PubMed: 26372384] |
| the journal of rheumatology | Biomarkers in Remission According to Different Criteria in Patients with Rheumatoid Arthritis [PubMed: 26472417] |
| Int J Mol Med | Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway [PubMed: 26677102] |
| the journal of biological chemistry | Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast Function [PubMed: 25837253] |
| frontiers in endocrinology | Oxytocin reverses osteoporosis in a sex-dependent manner [PubMed: 26042090] |
| PLoS One.? | The RIP1–RIP3 Complex Mediates Osteocyte Necroptosis after Ovariectomy in Rats [pmc:PMC4795547] |
| J Cell Physiol | Lanthanum Chloride Attenuates Osteoclast Formation and Function Via the DownreCavia (Guinea pig )lation of Rankl-Induced Nf-κb and Nfatc1 Activities [Pubmed:26060084] |
| FASEB Journal | N-Homocysteinylation impairs collagen cross-linking in cystathionine β-synthase-deficient mice: a novel mechanism of connective tissue abnormalities. [pubmed:27530978] |
| Metabolism. | High FGF21 levels are associated with altered bone homeostasis in HIV-1-infected patients. [pubmed:28521869] |
| journal of science and medicine in sport | The effect of calcium and vitamin D supplementation on bone health of male Jockeys. [pubmed:27568072] |
| Chemico-Biological Interactions | Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway [pubmed:27387537] |
| Epilepsy?Research | The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats [pubmed:27838501] |
| Bone | Reduced femoral bone mass in both diet-induced and genetic hyperlipidemia mice [pubmed:27669658] |
| Journal?of?Vascular?Surgery | A?potential?role?for?glycated?cross-links?in?abdominal?aortic?aneurysm?disease. [pubmed:27624703] |
| Institute?of?Pharmacology | Effects?of?long-term?administration?of?pantoprazole?on?bone?mineral?density?in?young?male?rats. [pubmed:27552060] |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752] |
| European Journal of Pharmacology | Cannabidiol administration reduces sublesional cancellous bone loss in rats with severe spinal cord injury [pubmed:28479140] |
| APPLIED?SPECTROSCOPY | Side-Effects of Convulsive Seizures and Anti-Seizure Therapy on Bone in a Rat Model of Epilepsy [pubmed:28905646] |
| Clinical Nutrition | Obesity status influences the relationship among serum osteocalcin, iron stores and insulin sensitivity [pubmed:29050649] |
| Oncotarget | A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice. [pubmed:28060768] |
| 中國骨質(zhì)疏松雜志 | 骨形成指標(biāo)PINP 與骨吸收指標(biāo)β-CTX 在2 型糖尿病合并骨質(zhì)疏松中作用的研究. [10.3969/j.issn.1006-7108.2017.03.009] |
| BioMed Research International | Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research [pubmed:29119115] |
| Cell?Death?And Differentiation | TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 [Pubmed:29358671] |
| Journal of Periodontology? | The role of androgens on periodontal repair in female rats [Pubmed:29683499] |
| Life?Sciences | Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway [Pubmed:29883721] |
| European?Journal of Pharmacology | The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism [Pubmed:29428468] |
| Journal of Clinical Densitometry | Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study [Pubmed:30076009] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457] |
| Experimental and Therapeutic Medicine | Liraglutide exerts a bone?protective effect in ovariectomized rats with streptozotocin?induced diabetes by inhibiting osteoclastogenesis [Pubmed:29805533] |
| Experimental and Therapeutic Medicine | Combination therapy with BMP?2 and psoralen enhances fracture healing in ovariectomized mice [10.3892:etm.2018.6353] |
| Tumor Biology | Dual PI3 K/mTOR inhibition reduces prostate cancer bone engraftment altering tumor-induced bone remodeling [Pubmed:29687745] |
| Sensors | Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers [Pubmed: 30181433] |
| Tropical Journal of Pharmaceutical Research | Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway [] |
| Bone?&?Joint?Research | Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise [] |
| Toxicol Ind Health | Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed?… [Pubmed: 30983556] |
| Journal of Geriatric?Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517] |
| Scientific?Reports | Significance of the Tks4 scaffold protein in bone tissue homeostasis [] |
| E The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats [] | |
| Biology Open | Osteocyte TSC1 promotes sclerostin secretion to restrain osteogenesis in mice [Pubmed: 31088250] |
| Journal of Periodontology | Role of testosterone and androgen receptor in periodontal disease progression in female rats [Pubmed: 31389012] |
| Journal of clinical periodontology | Biomarkers of oral inflammation in perinatally HIV‐infected and perinatally HIV exposed, uninfected youth [Pubmed: 31385616] |
| Bulletin of Experimental Biology and Medicine | Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone Graft [Pubmed: 31630302] |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities [Pubmed: 31739209] |
| International Journal of Environmental Research and Public Health | Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats [Pubmed: 31739465] |
| International journal of molecular sciences | Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients [Pubmed: 31717649] |
| Brazilian Journal of Medical and Biological Research | Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals [Pubmed: 31826180] |
| Journal of Bone and Mineral Research | BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling [Pubmed: 31914211] |
| Theranostics | EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for Osteoporosis [Pubmed: 32194868] |
| Cells | Article Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous?… [Pubmed: 31877673] |
| Cell | RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis [Pubmed: 32640190] |
| Advanced Science | A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype … [Pubmed: 32670749] |
| International Medical Journal Malaysia | The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats. [] |
| Cells. | Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous?… [] |
| Journal of Orthopaedic Translation | Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats [] |
| Research square | 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice [] |
| IIUM Medical Journal Malaysia | The Effects of Ficus Carica Fruit on Bone Markers and Oestrogen Level of Post-Menopausal Osteoporotic Rats [] |
| 淫羊藿水提物及提取藥渣的抗骨質(zhì)疏松藥效比較研究 [] | |
| Pamukkale T?p Dergisi | Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza ba?l? k?r?k riskinin ve kemik Turnoveri'nin de?erlendirilmesi [] |
| Science Advances | A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair [33310848] |
| J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/|??\catenin pathway [33950533] |
| Br J Pharmacol | Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis [34233019] |
| PHARMACEUTICAL BIOLOGY | Effects of icariin on the fracture healing in young and old rats and its mechanism [34511043] |
| Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway [34512780] |
| Scandinavian Cardiovascular Journal | Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy [34296634] |
| Chronobiol Int | Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in Poland [Pubmed:35139709] |
| Cell Metab | Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression [Pubmed:35235775] |
| Cell Death Discov | BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis [Pubmed:35523787] |
| Experimental and Molecular Medicine | Foxf2 represses bone formation via Wnt2b/β-catenin signaling [Pubmed:35668101] |
| Bioactive Materials | Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles [Pubmed:35386445] |
| Journal of Orthopaedic Translation | Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathway [Pubmed:35228997] |
| Journal of Orthopaedic Translation | Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35?… [] |
留言咨詢
