胰高血糖素樣肽1(GLP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Glucagon Like Peptide 1 (GLP1)
- 編號UCEA804Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍12.35-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于4.94pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測胰高血糖素樣肽1(GLP1)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的胰高血糖素樣肽1(GLP1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-91 | 86 |
| EDTA plasma(n=5) | 93-101 | 96 |
| heparin plasma(n=5) | 88-96 | 91 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的胰高血糖素樣肽1(GLP1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中胰高血糖素樣肽1(GLP1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-99% | 79-95% | 78-92% | 80-98% |
| EDTA plasma(n=5) | 88-95% | 80-97% | 99-105% | 88-99% |
| heparin plasma(n=5) | 78-101% | 93-105% | 78-95% | 92-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質水平。將胰高血糖素樣肽1(GLP1)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經(jīng)洗滌去掉未結合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| UCEA804Ra | 胰高血糖素樣肽1(GLP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA804Ra | 抗胰高血糖素樣肽1抗體(Anti-GLP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| ULMA804Ra | 胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA804Ra11 | 胰高血糖素樣肽1(GLP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Diabetes, Obesity and Metabolism | Specificity and sensitivity of commercially available assays for glucagon‐like peptide‐1 (GLP‐1): implications for GLP‐1 measurements in clinical studies [Pubmed:25041349] |
| Acta Histochemica | Expression of cholecystokinin2-receptor in rat and human L cells and the stimulation of glucagon-like peptide-1 secretion by gastrin treatment [Pubmed:25601282] |
| Journal of Endocrinology | Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNS [Pubmed:25630331] |
| Obes Surg | Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model [PubMed: 26202420] |
| International Journal of Pharmacognosy and Phytochemical Research | Anti-hiperglycemic Effect of Urena lobata Leaf Extract by Inhibition of Dipeptidyl Peptidase IV (DPP-IV) on Diabetic Rats [Pdf: 7] |
| European Journal of Pharmacology | Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model [Pubmed:27131827] |
| Int J Obes | miR-200a reCavia (Guinea pig )lates Rheb-mediated amelioration of insulin resistance after duodenal-jejunal bypass [Pubmed:27121251] |
| Journal of Traditional and Complementary Medicine | Incretin effect of Urena lobata leaves extract on structure and function of rats islet β-cells [S2225411016302802] |
| World?Journal of Gastroenterology? | Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats [pubmed:27547013] |
| World?Journal of Gastroenterology | Effects?of?sleeve?gastrectomy?with?jejuno-jejunal?or?jejuno-ileal?loop?onglycolipid?metabolism?in?diabetic?rats. [pubmed:27621579] |
| European?Journal?of?Nutrition | Improvement?in?glucose?tolerance?and?insulin?sensitivity?by?probiotic?strains?of?Indian?gut?originin?high-fat?diet-fed?C57BL/6J?mice. [pubmed:27757592] |
| Journal of Traditional and Complementary Medicine? | Incretin effect of Urena lobata leaves extract on structure and function of rats islet b-cells [d69b5594c032d557c3f3203b91aba2604a94] |
| Metabolism | Deactivation of the NLRP3 inflammasome in infiltrating macrophages by duodenal-jejunal bypass surgery mediates improvement of beta cell function in type 2 diabetes [10.1016/j.metabol.2017.10.015] |
| Obesity surgery | The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats. [pubmed:28840471] |
| Obesity?Surgery | Selective Vagotomy Worsens Glucose Control After Ileal Transposition [Pubmed:29525935] |
| Obesity?Surgery? | Preserving Duodenal-Jejunal (Foregut) Transit Does Not Impair Glucose Tolerance and Diabetes Remission Following Gastric Bypass in Type 2 Diabetes Sprague?… [Pubmed:29098544] |
| Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555] |
| Journal of Physiology and Biochemistry | Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat model [Pubmed:29781038] |
| Metabolism | Deactivation of the NLRP3 inflammasome in infiltrating macrophages by duodenal-jejunal bypass surgery mediates improvement of beta cell function in type 2?… [Pubmed:29129820] |
| Nutrition in the Prevention and Treatment of Abdominal Obesity | Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig [] |
| Peptides | The influence of high fat diet on plasma incretins and insulin concentrations in Sprague-Dawley rats with diet-induced obesity and glucose intolerance undergoing?… [Doi: 10.1016/j.peptides.2019.04.001] |
| The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811] |
| Neuropsychobiology | Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol Dependence [Pubmed: 31117084] |
| OBESITY SURGERY | The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in Rats [Pubmed: 31701411] |
| Journal of Lipid Research | Novel GPR120 agonist TUG891 modulates fat taste perception and preference and activates tongue-brain-gut axis in mice [Pubmed: 31806728] |
| Metabolik sendromlu s??anlarda yüzme egzersizinin plazma glukagon-benzeri PEPT?D-1 (GLP-1) seviyesi ve hipokampal GLP-1 resept?rü üzerine etkisinin?… [] | |
| World J Diabetes | Effect of oligofructose on resistance to postoperative high-fat diet-induced damage of metabolism in diabetic rats after sleeve gastrectomy [33889290] |
| Study on the Therapeutic Effects of Traditional Chinese Medicine on Insulin Resistance in Obese Mice by Modulating Intestinal Function [] | |
| Am J Physiol Endocrinol Metab | Central GLP-1 contributes to improved cognitive function and brain glucose uptake after duodenum-jejunum bypass on obese and diabetic rats [34370593] |
| Surgical Endoscopy | The effect of bariatric surgery on the expression of Growth Differentiation Factor-15/Macrophage-Inhibitory Cytokine-1 (GDF-15/MIC-1) in rat [Pubmed:35029766] |
| Sci Rep | The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairment [Pubmed:35468904] |
| Nutr Diabetes | Response of blood glucose and GLP-1 to different food temperature in normal subject and patients with type 2 diabetes [Pubmed:35624116] |
| Different Estradiol Level's Impact on Appetite Regulation, Food Intake, and Concentrations of Leptin, Glucagon-Like Peptide-1, and High-Sensitivity C-Reactive?… [] |
