抗三碘甲狀腺原氨酸抗體(Anti-T3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Anti-Triiodothyronine Antibody (Anti-T3)
3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin
- 編號(hào)UAEA453Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法間接法
- 反應(yīng)時(shí)長2h, 30min
- 檢測(cè)范圍3.12-200ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于1.27ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1785 ¥ 2550 ¥ 11475 ¥ 21675 ¥ 178500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)抗三碘甲狀腺原氨酸抗體(Anti-T3)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的抗三碘甲狀腺原氨酸抗體(Anti-T3)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-94 | 81 |
| EDTA plasma(n=5) | 93-102 | 98 |
| heparin plasma(n=5) | 80-96 | 90 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的抗三碘甲狀腺原氨酸抗體(Anti-T3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中抗三碘甲狀腺原氨酸抗體(Anti-T3)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-92% | 80-98% | 88-99% | 79-103% |
| EDTA plasma(n=5) | 96-105% | 88-102% | 88-97% | 78-105% |
| heparin plasma(n=5) | 92-103% | 94-105% | 78-97% | 95-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100μL，37°C孵育1小時(shí)；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分鐘；
6. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將抗原包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的抗三碘甲狀腺原氨酸抗體(Anti-T3)與連接于固相載體上的抗原結(jié)合，然后加入HRP標(biāo)記的二抗，徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的抗三碘甲狀腺原氨酸抗體(Anti-T3)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA453Ge11 | 三碘甲狀腺原氨酸(T3)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA453Ge21 | 三碘甲狀腺原氨酸(T3)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA453Ge01 | 三碘甲狀腺原氨酸(T3)多克隆抗體 | ELISA |
| ULAA453Ge71 | 三碘甲狀腺原氨酸(T3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA453Ge21 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA453Ge23 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA453Ge22 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA453Ge24 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA453Ge25 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA453Ge27 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | WB; IHC; ICC; IP. |
| ULAA453Ge72 | 三碘甲狀腺原氨酸(T3)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UCEA453Ge | 三碘甲狀腺原氨酸(T3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA453Ge | 三碘甲狀腺原氨酸(T3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA453Ge | 三碘甲狀腺原氨酸(T3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA453Ge | 抗三碘甲狀腺原氨酸抗體(Anti-T3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| UCCA453Ge | 三碘甲狀腺原氨酸(T3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA453Ge | 三碘甲狀腺原氨酸(T3)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA453Ge11 | 三碘甲狀腺原氨酸(T3)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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