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玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

試劑盒中的抗體對玻連蛋白(VTN)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

雜志	參考文獻
Biomedical Engineering and Informatics	Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563]
PLoS ONE	Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance [PubMed: PMC3754973]
Liver International	Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE [Pubmed: 23944848]
Analytical Biochemistry	A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots [Pubmed:29154788]
Journal of Materials Chemistry B	Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs [Doi: 10.1039/C8TB02283F?]
JOURNAL OF NEPHROLOGY	Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts [33275196]

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編號	適用物種：Rattus norvegicus (Rat，大鼠)	應用(僅供研究使用，不用于臨床診斷！)
URPB041Ra01	玻連蛋白(VTN)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UAPB041Ra01	玻連蛋白(VTN)活性蛋白	Cell?culture;?Activity?Assays.
UPAB041Ra01	玻連蛋白(VTN)多克隆抗體	WB; IHC
UMAB041Ra21	玻連蛋白(VTN)單克隆抗體	WB; IHC; ICC; IP.
USEB041Ra	玻連蛋白(VTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
ULMB041Ra	玻連蛋白(VTN)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSB041Ra01	玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".