孕酮(PG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Progesterone (PG)
P4; Pregn-4-Ene-3,20-Dione
- 編號(hào)UCCA459Ge
- 物種Pan-species (General，通用) 相同的名稱(chēng)，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)2h
- 檢測(cè)范圍1.2-300ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.35ng/mL.
- 樣本類(lèi)型serum, plasma and other biological fluids
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1714 ¥ 2448 ¥ 11016 ¥ 20808 ¥ 171360
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)孕酮(PG)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的孕酮(PG)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 93-105 | 101 |
| heparin plasma(n=5) | 80-96 | 84 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的孕酮(PG)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中孕酮(PG)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-102% | 95-102% | 83-104% | 98-105% |
| EDTA plasma(n=5) | 95-105% | 98-105% | 96-104% | 96-103% |
| heparin plasma(n=5) | 80-104% | 79-95% | 82-97% | 96-105% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測(cè)液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分鐘；
7. 讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競(jìng)爭(zhēng)抑制酶聯(lián)免疫分析法測(cè)定標(biāo)本中待測(cè)物質(zhì)水平。將孕酮(PG)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測(cè)抗原（標(biāo)準(zhǔn)品或樣本），待測(cè)抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競(jìng)爭(zhēng)結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會(huì)產(chǎn)生輝光型光信號(hào)。發(fā)射光強(qiáng)度和樣品中的孕酮(PG)呈負(fù)相關(guān)。用化學(xué)發(fā)光儀測(cè)定相對(duì)光單位（RLU），計(jì)算樣品濃度。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA459Ge31 | 孕酮(PG)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA459Ge11 | 孕酮(PG)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA459Ge21 | 孕酮(PG)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA459Ge41 | 孕酮(PG)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA459Ge01 | 孕酮(PG)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UPAA459Ge02 | 孕酮(PG)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAA459Ge71 | 孕酮(PG)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA459Ge24 | 孕酮(PG)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA459Ge21 | 孕酮(PG)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA459Ge23 | 孕酮(PG)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UMAA459Ge22 | 孕酮(PG)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| UCEA459Ge | 孕酮(PG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA459Ge | 孕酮(PG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA459Ge | 孕酮(PG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA459Ge | 孕酮(PG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA459Ge11 | 孕酮(PG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Fertility and Sterility | Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells [Pubmed: 23954356] |
| Am J Obstet Gynecol. | Prevention of preterm birth by progestational agents: what are the molecular mechanisms? [Pubmed: 23433326] |
| Placenta | The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cells [Pubmed: 23966103] |
| Veterinary Immunology and Immunopathology | Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period [Pubmed:27090626] |
| Clinical and Experimental Hypertension | Assessment of ADMA, estradiol, and progesterone in severe preeclampsia [Pubmed:27152507] |
| Scientific Reports | The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization [pubmed:27876896] |
| Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373] |
| 33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389] |
| Toxicological?Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
| Molecular?Neurobiology | Middle-Aged?Diabetic?Females?and?Males?Present?Distinct?Susceptibility?to?Alzheimer?Disease-like?Pathology. [pubmed:27730513] |
| Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267] |
| Interact Cardiovasc Thorac Surg | Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats [pubmed:29049608] |
| Toxicological Sciences | Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666] |
| Life Sciences | The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats [pubmed:28599864] |
| Placenta | Progesterone attenuates hypertension and autoantibody levels to the angiotensin II type 1 receptor in response to elevated cadmium during pregnancy [10.1016:j.placenta.2017.12.004] |
| Nanotheranostics | The biocompatibility studies of polymer dots on pregnant mice and fetuses [pubmed:29071192] |
| DARU Journal of Pharmaceutical Sciences | Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999] |
| Toxicological?Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
| Medical?Science?Monitor | Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian?… [Pubmed:29674607] |
| Oncology Letters | Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes?… [10.3892:ol.2018.9165] |
| Biomedicine & Pharmacotherapy | Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons [Doi: 10.1016/j.biopha.2018.08.135] |
| Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146] |
| Stem?Cell?Research?&?Therapy | Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the?… [Pubmed: 30606243] |
| Journal of?Cellular?Physiology | Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression [Pubmed: 30671954] |
| Experimental and Therapeutic Medicine | Tanshinone IIA attenuates estradiol?induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary [] |
| Experimental Neurology | Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause [Pubmed: 31377402] |
| Molecular reproduction and development | Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries [Pubmed: 31490589] |
| Journal of Ovarian Research | Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718] |
| Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
| Biosensors & Bioelectronics | An antibody-aptamer sandwich cathodic photoelectrochemical biosensor for the detection of progesterone [Pubmed: 32339148] |
| JOURNAL OF MOLECULAR NEUROSCIENCE | Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment [Pubmed: 33159671] |
| Chemosphere | Prenatal exposure of female mice to per?uorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21?… [Pubmed: 33131727] |
| ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043] |
| The Journal of Physiology | Guoqi Liu1, Juan Li2, 3, Peng Cui2, Susanne Lager5, Amanda Nancy Sferruzzi-Perri6, Wei Li1, Xiao-ke Wu1, Yanhua Han1, Mats Br?nnstr?m7, Linus R Shao2?… [Pubmed: 31206177] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| DOMESTIC ANIMAL ENDOCRINOLOGY | Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells [Pubmed: 32739762] |
| Journal of Reproductive Immunology | Implantation associated changes in expression profile of indoleamine-2, 3-dioxygenase 1, Th1-Th2 cytokines and interferon-stimulated genes on neutrophils and?… [Pubmed: 32846354] |
| Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS [] |
| Improving reproductive efficiency through the supplementation of mustard oil, poly-herbal mixture and butyric acid during the periparturient period in Sahiwal?… [] | |
| Ecotoxicol Environ Safe | A preliminary study on the mechanism of the neurosteroid-mediated ionotropic receptor dysfunction in neurodevelopmental toxicity induced by??- [33862428] |
| Life Sciences | MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl [34273377] |
| Antioxidants (Basel) | Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer [34439574] |
| J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391] |
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