波形蛋白(VIM)多克隆抗體
Polyclonal Antibody to Vimentin (VIM)
- 編號UPAB040Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPB040Hu01-波形蛋白(VIM)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Equus caballus; Equine (Horse，馬), Chicken (Gallus，雞)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 299 ¥ 697 ¥ 996 ¥ 2490 ¥ 9960
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human VIM Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Uterus Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lymph node Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lung Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Pancreas Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Spleen Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Colon Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Spinal cord Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human VIM Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human VIM Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Spleen Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human VIM Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: A549 cell lysate; Primary Ab: 0.1μg/ml Rabbit Anti-Human VIM Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Rat Heart lysate; Lane3: Rat Cerebrum lysate; Lane4: 293T cell lysate; Lane5: Hela cell lysate
Primary Ab: 0.1μg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Hela cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Canine Heart lysate; Lane2: Porcine Heart lysate; Lane3: Bovine Heart lysate; Lane4: Caprine Heart lysate; Lane5: Equine Heart lysate; Lane6: Gallus Heart lysate; Lane7: Cavia Heart lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Rat Heart lysate; Lane3: 293T cell lysate; Lane4: Hela cell lysate
Primary Ab: 0.1μg/ml Rabbit Anti-Human VIM Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對VIM的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別VIM。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB040Hu02 | 波形蛋白(VIM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB040Hu01 | 波形蛋白(VIM)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB040Hu01 | 波形蛋白(VIM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPB040Hu61 | 波形蛋白(VIM)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPB040Hu62 | 波形蛋白(VIM)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB040Hu02 | 波形蛋白(VIM)多克隆抗體 | IHC; ICC/IF |
| UPAB040Hu01 | 波形蛋白(VIM)多克隆抗體 | WB; IHC; ICC/IF |
| ULAB040Hu71 | 波形蛋白(VIM)多克隆抗體(生物素標記) | WB |
| UMAB040Hu24 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB040Hu25 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB040Hu21 | 波形蛋白(VIM)單克隆抗體 | WB; IHC |
| UMAB040Hu23 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB040Hu27 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB040Hu22 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UFAB040Hu02 | 抗波形蛋白(VIM)單克隆抗體 | FCM |
| UFAB040Hu06 | 抗波形蛋白(VIM)單克隆抗體 | FCM |
| UFAB040Hu04 | 抗波形蛋白(VIM)單克隆抗體 | FCM |
| UFAB040Hu08 | 抗波形蛋白(VIM)單克隆抗體 | FCM |
| USEB040Hu | 波形蛋白(VIM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB040Hu | 波形蛋白(VIM)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB040Hu01 | 波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Cancer?Epidemiology,?Biomarkers?& Prevention | Aberrant Vimentin Methylation Is Characteristic of Breast Cancer [pubmed:22315367] |
| Scientific Reports | Glyoxalase?1-knockdown?in?human?aortic?endothelial?cells - effect on the?proteome?andendothelial?function?estimates. [pubmed:27898103] |
| Journal of?Proteomics | HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin [Pubmed:29477555] |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
| American Journal of Transplantation | Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts [Pubmed: 31729155] |
| Lung | Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? [Pubmed: 32088750] |
| Cell Biol Toxicol | ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer [33566221] |
| CELL BIOLOGY AND TOXICOLOGY | Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence [33758996] |
| Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women [] | |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] |
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