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血小板因子4(PF4)多克隆抗體

Polyclonal Antibody to Platelet Factor 4 (PF4)

CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

編號(hào)UPAA172Mu02
物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來(lái)源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無(wú)標(biāo)記物
免疫原 RPA172Mu02-血小板因子4(PF4)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度500μg/mL
且適物種Homo sapiens (Human，人)
應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。
下載英文說(shuō)明書中文說(shuō)明書
規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Mouse Lung Tissue Primary Ab: 10μg/ml Rabbit Anti-Mouse PF4 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Spleen Tissue
Primary Ab: 10μg/ml Rabbit Anti-Mouse PF4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot Sample: Recombinant PF4, Mouse
Western Blot; Sample: Human Platelet lysate
Primary Ab: 1μg/ml Rabbit Anti-Mouse PF4 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Spleen lysate
Primary Ab: 1μg/ml Rabbit Anti-Mouse PF4 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)PF4的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別PF4。

用法

Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics	Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats [PubMed: 20601223]
Basic Research in Cardiology	Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170]
PLoS One	Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation [PubMed: PMC3608633]
Diving and Hyperbaric Medicine	Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression [Pubmed:25311322]
Journal of Functional Foods	A?Phellinus baumii–based supplement containing?Salvia miltiorrhiza?Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro?and?in vivo [science:S1756464616300676]
Plos one	Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury [pubmed:27583400]
international journal of molecular sciences	Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. [pubmed:28272338]
Experimental?and?Therapeutic?Medicine	Feasibility of improving platelet?rich plasma therapy by using chitosan with high platelet activation ability [pubmed:28450960]
Reproductive Toxicology	Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats [Pubmed: 30528429]
INTERNATIONAL JOURNAL OF CARDIOLOGY	Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation [Pubmed: 30857843]
Available at SSRN 3335365?	Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers []
journal of biotechnology	Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis [Pubmed: 30927490]
Data in Brief	Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation []
journal of thoracic and cardiovascular surgery	Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers [Pubmed: 32812629]
Marine Drugs	The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230]
Thrombosis Research	Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449]
Nat Commun	The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets [34099640]
Vaccine	The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388]

留言咨詢

編號(hào)	適用物種：Mus musculus (Mouse，小鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
UAPA172Mu01	血小板因子4(PF4)活性蛋白	Cell?culture;?Activity?Assays.
URPA172Mu01	血小板因子4(PF4)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
URPA172Mu02	血小板因子4(PF4)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA172Mu01	血小板因子4(PF4)多克隆抗體	WB; IHC; ICC; IP.
UPAA172Mu02	血小板因子4(PF4)多克隆抗體	WB; IHC
ULAA172Mu71	血小板因子4(PF4)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
ULAA172Mu81	血小板因子4(PF4)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
USEA172Mu	血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
USCA172Mu	血小板因子4(PF4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
ULMA172Mu	血小板因子4(PF4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA172Mu01	血小板因子4(PF4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".