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雌激素受體β(ERb)單克隆抗體

Monoclonal Antibody to Estrogen Receptor Beta (ERb)

ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3,Group A,Member 2

編號(hào)UMAA437Hu22
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))-
Ig亞型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 -
緩沖液成份磷酸鹽緩沖液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性狀液體
濃度1mg/mL
且適物種-
應(yīng)用WB; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
下載英文說明書中文說明書
規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

AF488 staining on IF;
Sample: U2OS cell
Primary Ab: 20μg/ml Mouse Anti-Human ERb Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)

Western Blot; Samples: Lane1: MCF7 cell lysate; Lane2: PC3 cell lysate;
Primary Ab: 0.01μg/ml Mouse Anti-Human ERb Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)ERb的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別ERb。

用法

免疫印跡：0.2-2μg/mL;1:500-5000
免疫組織化學(xué)：5-20μg/mL;1:50-200
免疫細(xì)胞化學(xué)：5-20μg/mL;1:50-200
最佳稀釋倍數(shù)最終由用戶決定。

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽(yáng)性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長(zhǎng)臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
The Journal of Steroid Biochemistry and Molecular Biology	Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829]
Biochem Biophys Res Commun	Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas [PubMed: 26529545]
Mol Neurobiol	Selective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against Hypoxia [PubMed: 26476840]
J Steroid Biochem Mol Biol	Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR31 signalling [PubMed: 24846830]
Journal of Environmental Sciences	Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924]
Biol Open.	Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine. [pubmed:27875242]
Biomedicine & Pharmacotherapy	Postmenopausal osteoporosis is associated with the regulation of SP, CGRP, VIP, and NPY [Pubmed:29807224]
Journal of Applied Pharmaceutical Science	Physical Signs of Illness, Liver Functions and Reproductive Parameters of Female Rats Supplemented with Melaleuca cajuputi Methanolic Extract [Pbulication:325682424]
Carleton University Research Virtual Environment	In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts []
Life Sciences	MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl [34273377]
Cardiometry	Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue []

留言咨詢

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA437Hu01	雌激素受體β(ERb)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
UPAA437Hu01	雌激素受體β(ERb)多克隆抗體	WB; ICC/IF
ULAA437Hu71	雌激素受體β(ERb)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
ULAA437Hu81	雌激素受體β(ERb)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
UFAA437Hu11	抗雌激素受體β(ERb)多克隆抗體(標(biāo)記)	FCM
UMAA437Hu22	雌激素受體β(ERb)單克隆抗體	WB; ICC/IF
UMAA437Hu21	雌激素受體β(ERb)單克隆抗體	WB; ICC/IF
USEA437Hu	雌激素受體β(ERb)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
USCA437Hu	雌激素受體β(ERb)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
ULMA437Hu	雌激素受體β(ERb)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
UKSA437Hu01	雌激素受體β(ERb)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".