載脂蛋白B(APOB)單克隆抗體
Monoclonal Antibody to Apolipoprotein B (APOB)
Apo-B; FLDB; Apo B-100
- 編號UMAC003Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C3
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 NPC003Hu01-載脂蛋白B(APOB)天然蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; ICC/IF; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 317 ¥ 739 ¥ 1056 ¥ 2640 ¥ 10560
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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IHC圖
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AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 30μg/ml Mouse Anti-Human APOB Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Figure. Western Blot; Sample: Recombinant APOB, Human.
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Western Blot; Sample: Human Serum;
Primary Ab: 3μg/ml Mouse Anti-Human APOB Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Human Hepa1-6 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human APOB Monoclonal Antibody (Catalog MAC003Hu22, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對APOB的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別APOB。
用法
Western blotting: 0.01-3μg/mL;
Immunocytochemistry: 5-30μg/mL;
Flow cytometry: 10μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPC003Hu01 | 載脂蛋白B(APOB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC003Hu02 | 載脂蛋白B(APOB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC003Hu01 | 載脂蛋白B(APOB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAC003Hu02 | 載脂蛋白B(APOB)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAC003Hu01 | 載脂蛋白B(APOB)多克隆抗體 | WB |
| ULAC003Hu81 | 載脂蛋白B(APOB)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAC003Hu71 | 載脂蛋白B(APOB)多克隆抗體(生物素標(biāo)記) | WB |
| UMAC003Hu24 | 載脂蛋白B(APOB)單克隆抗體 | IHC |
| UMAC003Hu25 | 載脂蛋白B(APOB)單克隆抗體 | IHC |
| UMAC003Hu23 | 載脂蛋白B(APOB)單克隆抗體 | WB; IHC |
| UMAC003Hu22 | 載脂蛋白B(APOB)單克隆抗體 | WB; ICC/IF; FCM |
| UMAC003Hu21 | 載脂蛋白B(APOB)單克隆抗體 | WB |
| UMAC003Hu26 | 載脂蛋白B(APOB)單克隆抗體 | WB; IHC; ICC; IP. |
| UFAC003Hu06 | 抗載脂蛋白B(APOB)單克隆抗體 | FCM |
| UFAC003Hu04 | 抗載脂蛋白B(APOB)單克隆抗體 | FCM |
| UFAC003Hu02 | 抗載脂蛋白B(APOB)單克隆抗體 | FCM |
| UFAC003Hu08 | 抗載脂蛋白B(APOB)單克隆抗體 | FCM |
| USEC003Hu | 載脂蛋白B(APOB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEC003Hu | 載脂蛋白B(APOB)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEC003Hu | 抗載脂蛋白B抗體(Anti-APOB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCC003Hu | 載脂蛋白B(APOB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMC003Hu | 載脂蛋白B(APOB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Lipid Research | Group 1B phospholipase A2 deficiency protects against diet-induced hyperlipidemia in mice [Jlr: M019463] |
| J Clin Endocrinol Metab.? | Elevated retinol binding protein 4 induces apolipoprotein B production and associates with hypertriglyceridemia [Pubmed:25781360] |
| Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253] |
| Am J Cardiovasc Dis | Hypoascorbemia induces atherosclerosis and vascular deposition of lipoprotein (a) in transgenic mice [PubMed: 26064792] |
| The Journal of Biological Chemistry | Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation [Pubmed:27284008] |
| United States Patent Application | COMPOSITIONS AND METHODS FOR EFFICACIOUS AND SAFE DELIVERY OF SIRNA USING SPECIFIC CHITOSAN-BASED NANOCOMPLEXES [y2016:0152988.html] |
| alcohol and alcoholism | Hops (Humulus lupulus) Content in Beer Modulates Effects of Beer on the Liver After Acute Ingestion in Female Mice. [pubmed:27659607] |
| Frontiers in Physiology | Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats [pubmed:28798692] |
| Food?Research International | Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541] |
| Journal of The Science of Food and Agriculture | Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773] |
| European?Journal of Pharmacology | The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981] |
| Nature Communications | Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794] |
| Study of in Vivo Serum Lipid Regulation with Ulmus macrocarpa Hance Extract in Rats [] | |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
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