基質(zhì)金屬蛋白酶9(MMP9)活性蛋白
Active Matrix Metalloproteinase 9 (MMP9)
GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase
- 編號UAPA553Mu61
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點5.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1010 ¥ 2526 ¥ 5052 ¥ 15156 ¥ 37890
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Mechanism:MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family . It is a gelatinase A, 92 kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10 pg of MMP-2 can already be detected .Briefly，various concentrations of recombinant mouse MMP9 (100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.1ng, 1.5ng and 0.7ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 15% gels) containing gelatin (1 mg/ml) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP9 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use trypsin as positive control.
Result: Gelatin hydrolysis by recombinant mouse MMP9(10-70kd) was shown in figure 1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA553Mu51 | 基質(zhì)金屬蛋白酶9(MMP9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA553Mu01 | 基質(zhì)金屬蛋白酶9(MMP9)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA553Mu61 | 基質(zhì)金屬蛋白酶9(MMP9)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA553Mu62 | 基質(zhì)金屬蛋白酶9(MMP9)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA553Mu62 | 基質(zhì)金屬蛋白酶9(MMP9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA553Mu61 | 基質(zhì)金屬蛋白酶9(MMP9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA553Mu01 | 基質(zhì)金屬蛋白酶9(MMP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA553Mu02 | 基質(zhì)金屬蛋白酶9(MMP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA553Mu02 | 基質(zhì)金屬蛋白酶9(MMP9)多克隆抗體 | WB; IHC |
| UPAA553Mu01 | 基質(zhì)金屬蛋白酶9(MMP9)多克隆抗體 | WB; ICC/IF |
| UPAA553Mu07 | 基質(zhì)金屬蛋白酶9(MMP9)多克隆抗體 | IHC |
| ULAA553Mu71 | 基質(zhì)金屬蛋白酶9(MMP9)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA553Mu | 基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA553Mu | 抗基質(zhì)金屬蛋白酶9抗體(Anti-MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCA553Mu | 基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA553Mu | 基質(zhì)金屬蛋白酶9(MMP9)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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| Turkish Journal of Biochemistry-Turk Biyokimya Dergisi | Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes [] |
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