白介素7受體(IL7R)活性蛋白
Active Interleukin 7 Receptor (IL7R)
CDW127; CD127; IL-7R-Alpha
- 編號UAPB905Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)5.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Interleukin 7 Receptor (IL7R), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation and survival. Besides, IL7R?is involved in the pathogenesis of neurodegenerative disorders, such as multiple sclerosis. Thymic Stromal Lymphopoietin (TSLP) has been identified as an interactor of IL7R, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IL7R and recombinant human TSLP. Briefly, biotin-linked IL7R were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to TSLP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of IL7R and TSLP was shown in Figure 1, the EC50?for this effect is 0.956 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPB905Hu61 | 白介素7受體(IL7R)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB905Hu01 | 白介素7受體(IL7R)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB905Hu61 | 白介素7受體(IL7R)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPB905Hu62 | 白介素7受體(IL7R)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB905Hu01 | 白介素7受體(IL7R)多克隆抗體 | WB; IHC; ICC/IF |
| UFAB905Hu41 | 抗白介素7受體(IL7R)多克隆抗體(藻紅素標(biāo)記) | FCM |
| UMAB905Hu27 | 白介素7受體(IL7R)單克隆抗體 | WB |
| UMAB905Hu24 | 白介素7受體(IL7R)單克隆抗體 | IHC |
| UMAB905Hu25 | 白介素7受體(IL7R)單克隆抗體 | IHC |
| UMAB905Hu28 | 白介素7受體(IL7R)單克隆抗體 | WB |
| UMAB905Hu21 | 白介素7受體(IL7R)單克隆抗體 | WB |
| UMAB905Hu23 | 白介素7受體(IL7R)單克隆抗體 | WB; IHC |
| UMAB905Hu26 | 白介素7受體(IL7R)單克隆抗體 | WB |
| URAB905Hu23 | 白介素7受體(IL7R)重組抗體 | ICC/IF; FCM |
| UMAB905Hu22 | 白介素7受體(IL7R)單克隆抗體 | WB |
| USEB905Hu | 白介素7受體(IL7R)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB905Hu | 白介素7受體(IL7R)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
