谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白
Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- 編號(hào)UAPB090Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)7.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Recombinant Glutathione S Transferase Pi (GSTp) is a key enzyme in the body. It conjugates glutathione with electrophilic substances, playing a vital role in detoxification and antioxidant defense. GSTp is involved in protecting cells from harmful substances and oxidative stress by binding with PRDX6 .Thus a functional ELISA assay was conducted to detect the interaction of recombinant rat GSTp and recombinant rat PRDX6. Briefly, GSTp was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PRDX6-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat GSTp and recombinant rat PRDX6 was shown in Figure 1, the EC50?for this effect is 0.008ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB090Ra01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB090Ra01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAB090Ra01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體 | WB; IHC |
| ULAB090Ra71 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體(生物素標(biāo)記) | WB |
| USEB090Ra | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB090Ra | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB090Ra01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117] |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Neuroscience?Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218] |
| Arabian Journal of Chemistry | Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats [] |
| International Journal of Nature and Life Sciences | The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands [] |
