補(bǔ)體成分1q子成分C(C1qC)活性蛋白
Active Complement Component 1, Q Subcomponent C (C1qC)
C1QG; Complement Component 1 q subcomponent,Gamma Polypeptide
- 編號UAPE869Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)7.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 778 ¥ 1944 ¥ 3888 ¥ 11664 ¥ 29160
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
Figure. SDS-PAGE
-
WB圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Complement component 1Q subcomponent C (C1qC) is an important part of the C1 complex in the classical activation pathway of the complement system. C1qC contains multiple domains that recognize and bind to pathogen surfaces or antibody-antigen complexes.C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. Thus a functional ELISA assay was conducted to detect the interaction of recombinant human C1qC and recombinant mouse C1r. Briefly, C1qC was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to C1r-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C1qC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human C1qC and recombinant mouse C1r was shown in Figure 1, the EC50?for this effect is 0.049ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPE869Hu01 | 補(bǔ)體成分1q子成分C(C1qC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPE869Hu01 | 補(bǔ)體成分1q子成分C(C1qC)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAE869Hu01 | 補(bǔ)體成分1q子成分C(C1qC)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAE869Hu21 | 補(bǔ)體成分1q子成分C(C1qC)單克隆抗體 | WB; IHC; ICC; IP. |
| USEE869Hu | 補(bǔ)體成分1q子成分C(C1qC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCE869Hu | 補(bǔ)體成分1q子成分C(C1qC)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULME869Hu | 補(bǔ)體成分1q子成分C(C1qC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLOS ONE | Increased complement C1q level marks active disease in human tuberculosis [Pubmed:24647646] |
