心肌肌鈣蛋白I(cTnI)活性蛋白
Active Cardiac Troponin I (cTnI)
TNNI3; TNNC1; CMH7; C-TnI; Troponin I Type 3; Troponin I Type 3, Cardiac
- 編號(hào)UAPA478Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)10.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià)
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Cardiac Troponin I (cTnI), as a cytoplasmic protein, is one of the most important regulatory proteins part of the troponin-tropomyosin complex localized on the actin (thin) myofilaments in cardiac myocytes.It is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.Besides,Actin Alpha 1, Cardiac Muscle (ACTC1) has been identified as an interactor of cTnI, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human cTnI and recombinant human ACTC1. Briefly, biotin-linked cTnI were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to ACTC1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of cTnI and ACTC1 was shown in Figure 1, the EC50?for this effect is 0.066ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA478Hu02 | 心肌肌鈣蛋白I(cTnI)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA478Hu61 | 心肌肌鈣蛋白I(cTnI)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA478Hu01 | 心肌肌鈣蛋白I(cTnI)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA478Hu01 | 心肌肌鈣蛋白I(cTnI)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA478Hu61 | 心肌肌鈣蛋白I(cTnI)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA478Hu01 | 心肌肌鈣蛋白I(cTnI)多克隆抗體 | WB; IHC |
| ULAA478Hu71 | 心肌肌鈣蛋白I(cTnI)多克隆抗體(生物素標(biāo)記) | WB |
| UMAA478Hu28 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| URAA478Hu21 | 心肌肌鈣蛋白I(cTnI)重組抗體 | WB |
| UMAA478Hu21 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| UMAA478Hu27 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| UMAA478Hu23 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB |
| UMAA478Hu24 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| UMAA478Hu25 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| UMAA478Hu29 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB |
| UMAA478Hu22 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB; IHC |
| UMAA478Hu26 | 心肌肌鈣蛋白I(cTnI)單克隆抗體 | WB |
| UFAA478Hu08 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | FCM |
| UFAA478Hu06 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | FCM |
| UFAA478Hu04 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | FCM |
| UFAA478Hu02 | 抗心肌肌鈣蛋白I(cTnI)單克隆抗體 | FCM |
| ULAA478Hu74 | 心肌肌鈣蛋白I(cTnI)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA478Hu | 心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA478Hu | 心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA478Hu | 抗心肌肌鈣蛋白I抗體(Anti-cTnI)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCA478Hu | 心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA478Hu | 心肌肌鈣蛋白I(cTnI)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA478Hu01 | 心肌肌鈣蛋白I(cTnI)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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